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| Effects of Sanjiao Qushi Formula on ZO-1 and synaptopodin protein expression in mice with membranous nephropathy |
| Hits 517 Download times 387 Received:January 08, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.05.22 |
| Key Words
Sanjiao Qushi Formula;membranous nephropathy;experimental research;ZO-1;synaptopodin |
| Author Name | Affiliation | E-mail | | CHEN Jingjie | Hebei University of Chinese Medicine, Hebei Key Laboratory of Integrative Medicine of Liver-Kidney Patterns, Shijiazhuang 050091, China | | | FANG Jing | Hebei University of Chinese Medicine, Hebei Key Laboratory of Integrative Medicine of Liver-Kidney Patterns, Shijiazhuang 050091, China | | | ZHAO Yayun | Hebei University of Chinese Medicine, Hebei Key Laboratory of Integrative Medicine of Liver-Kidney Patterns, Shijiazhuang 050091, China
The First Affiliated Hospital of Hebei University of Traditional Chinese Medicine, Shijiazhuang 050011, China | | | LIU Haiping | Hebei University of Chinese Medicine, Hebei Key Laboratory of Integrative Medicine of Liver-Kidney Patterns, Shijiazhuang 050091, China | | | MA Yun | Hebei University of Chinese Medicine, Hebei Key Laboratory of Integrative Medicine of Liver-Kidney Patterns, Shijiazhuang 050091, China
The First Affiliated Hospital of Hebei University of Traditional Chinese Medicine, Shijiazhuang 050011, China | | | CHEN Zhiqiang | The First Affiliated Hospital of Hebei University of Traditional Chinese Medicine, Shijiazhuang 050011, China | chenzhqiang2011@163.com |
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| Abstract
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| [Objective] To investigate the protective effect of Sanjiao Qushi Formula on renal tissueand the expression oftight junction protein ZO-1 andsynaptopodin in membranous nephropathy mice.[Methods] Sixty female Balb/c mice were randomly divided into control group (N group) (n=10) and modeling group (n=50). After 2 weeks of pre-immunization,formal immunization began.Cationized bovine serum albumin(C-BSA)(6.5 mg/kg) was injected by tail vein every other day for 6 weeks to replicate the mouse model of membranous nephropathy.After 6 weeks,the 24-hour urine protein quantification (24h-UTP) of all mice in the modeling group was positive,indicating that the modeling was successful.Four mice were randomly selected for renal pathology.Thickening of basement membrane accompanied by deposition of immune complex was observed,confirming the successful modeling of membranous nephropathy mouse model.The model group was randomly divided into model group (M group) (n=10),Chinese medicine low-dose group (L group) (n=10),Chinese medicine high-dose group (H group) (n=10) and Benazepril hydrochloride group (X group) (n=10). N group and M group were given 0.2 mL normal saline intragaedally.The high and low dose group of Sanjiao Qushi Formula and Benazepril hydrochloride group were treated with corresponding drugs (3.71 g/kg in group L,7.42 g/kg in group H and 1.3 mg/kg in group X),once a day,for 4 weeks. After the experiment,the mice were anesthetized,abdominal aorta blood was collected,and blood samples were collected to detect total cholesterol(TC),serum albumin (Alb),serum creatinine (Scr) and urea nitrogen (BUN).The pathological morphology of renal tissue was observed under light microscope.Immunofluorescence was used to detect the deposition of immunoglobulin G(IgG) in renal tissues. The expression levels of ZO-1 and synaptopodin in renal tissues were detected by Western blot.[Results] Compared with N group,24 h-UTP and TCin M group were significantly increased (P<0.05),Alb was significantly decreased (P<0.05);Compared with M group,24 h-UTP and TCin treatment groups were significantly decreased (P<0.05),and Alb was significantly increased (P<0.05);There were no significant differences in 24 h-UTP,TC and Albamong all treatment groups (P>0.05).There were nosignificant differences in Scr and BUN among all groups (P>0.05).Compared with N group,the expressions of ZO-1 and synaptopodin in renal tissues of M group were significantly decreased (P<0.05);Compared with M group,the expressions of ZO-1 and synaptopodin in kidney tissues of all treatment groups were significantly increased (P<0.05),but there were no significant differences in ZO-1 and synaptopodin expressions among all treatment groups (P>0.05).[Conclusion] Sanjiao Qushi Formula has protective effect on the kidney of membranous nephropathy mice,the mechanism may be related to the up-regulation of ZO-1 and synaptopodin protein expression. |
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