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| Study on the mechanism of Bushen Huoxue Recipe in the treatment of endometriosis based on PI3K/Akt/NF-κB pathway |
| Hits 488 Download times 395 Received:January 07, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.06.20 |
| Key Words
ndometriosis;Bushen Huoxue Recipe;PI3K/Akt/NF-κB signaling pathway;inflammatory reaction;CD31;VEGF;MMP-9 |
| Author Name | Affiliation | E-mail | | MA Zhongling | Department of Obstetrics, Qinghai Provincial People's Hospital, Xining 810007, China | | | LIU Xin | Department of Obstetrics, Qinghai Provincial People's Hospital, Xining 810007, China | 17195351107@163.com | | WANG Yufeng | Department of Obstetrics, Qinghai Provincial People's Hospital, Xining 810007, China | |
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| Abstract
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| Objective To explore the effect and mechanism of Bushen Huoxue Recipe (BSHXR) on endometriosis (EMS).Methods The EMS rat model was established by autologous endometrial transplantation. The rats were randomly divided into sham operation group, model group, BSHXR high, medium and low dose groups, LY294002 (PI3K inhibitor) group, with 10 rats in each group. After 28 days of administration, the volume of ectopic endometrium was measured. The histopathological changes of the endometrium were observed by HE staining. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in serum were detected by ELISA. The expression of platelet endothelial cell adhesion molecule-1(CD31) in ectopic endometrium was detected by immunohistochemistry. The mRNA levels of vascular endothelial growth factor(VEGF) and matrix metalloproteinase-9(MMP-9) in ectopic endometrium were detected by RT-qPCR. The protein expression of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/nuclear factor kappa-B (NF-κB) pathway in ectopic endometrium was detected by Western blot.Results Compared with sham operation group, the volume of ectopic endometrium in model group was significantly increased, the levels of serum inflammatory factors were increased, the number of CD31 positive cells was increased, the mRNA levels of VEGF and MMP-9 were increased, and PI3K/Akt/NF-κB signaling pathway was activated(P < 0.05). Compared with model group, BSHXR could reduce the volume of ectopic endometrium, decrease the serum levels of inflammatory factors, decrease the number of CD31 positive cells, decrease the expression levels of VEGF and MMP-9 mRNA, and inhibit the activation of PI3K/Akt/NF-κB signaling pathway in a dose-dependent manner (P < 0.05).Conclusion BSHXR has therapeutic effects on EMS in rats, and its mechanism may be related to the inhibition of PI3K/Akt/NF-κB signaling pathway activation. |
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