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| Effect and mechanism of Qiankun Dan Ⅵ on diabetic nephropathy model rats |
| Hits 583 Download times 441 Received:January 13, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.06.21 |
| Key Words
diabetic nephropathy;Qiankun Dan Ⅵ;CTGF;TGF-1β1;BMP-7 |
| Author Name | Affiliation | | LI Lianrui | Department of Pharmacy, Tianjin Beichen Hospitial of Traditional Chinese Medicine, Tianjin 300400, China | | CHEN Zhiyong | Department of Pharmacy, Tianjin Beichen Hospitial of Traditional Chinese Medicine, Tianjin 300400, China | | LIU Jie | Department of Pharmacy, Tianjin Beichen Hospitial of Traditional Chinese Medicine, Tianjin 300400, China | | LI Tianxiang | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
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| Abstract
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| Objective To investigate the therapeutic effect of Qiankundan Ⅵ on diabetic nephropathy (DN) and its possible mechanism, and to observe its effects on CTGF, TGF-β1 and BMP-7 in DN model rats.Methods By subcutaneous injection of STZ joint high sugar, high fat diet to the preparation of DN rats model, the model for the preparation of successful rats were randomly divided into model group and treatment group, and blank control group, giving medication, the dose after 12 weeks of detecting the blood sugar, the urine trace albumin, kidney CTGF, TGF-β1, BMP 7 expression level of rats by Western blot, the CTGF, TGF-β1, BMP 7 mRNA transcription level of rats by RT-PCR method.Results Compared with the blank group, blood glucose and urinary microalbumin in the model group were significantly increased, protein expression of CTGF and TGF-β1 and mRNA transcription were significantly up-regulated, protein expression of BMP-7 and mRNA transcription were significantly down-regulated. After treatment with Qiankun Dan Ⅵ, blood glucose, urinary trace albumin were significantly lower than those in the model group, protein expression and mRNA transcription level of CTGF and TGF-β1 were also significantly lower than those in the model group, and protein expression and mRNA transcription level of BMP-7 were significantly increased.Conclusion Qiankun Dan Ⅵ has therapeutic effects on DN rats, and its mechanism may be related to regulating TGF-β1-CTGF-BMP-7 axis. |
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