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| Simultaneous multicomponent profiling and characterization of five Qinggan Yin series preparations |
| Hits 516 Download times 480 Received:December 13, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.06.22 |
| Key Words
Qinggan Yin preparation;ultra-high performance liquid chromatography;ion mobility high-resolution mass spectrometry;HDMSE;efficient characterization |
| Author Name | Affiliation | E-mail | | SUN Mengxiao | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | DING Hui | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yuefei | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GAO Xiumei | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Xue | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | tjdxsyx@163.com;李雪，tjdxsyx@163.com | | YANG Wenzhi | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| Objective To analyze chemical constituents and synchronously profile the multiple components of Qinggan Yin series preparations (spring/summer/autumn/winter/children).Methods In this work, ultra-high performance liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry-based data-independent high-definition MSE (UHPLC/IM-QTOF-HDMSE) was established to achieve identification and elucidation of chemical constituents in Qinggan Yin series preparations comprehensively and rapidly. The chromatograms of Qinggan Yin series preparations and its single herbs involved were analyzed and compared under the same chromatography-MS conditions. The intelligentized and efficient characterization of Chinese medicine components could be achieved by automatic peak annotation workflows by UNIFITM and searching against the in-house libraries.Results A total of 177 compounds were identified or tentatively characterized from five Qinggan Yin preparations, including 73 flavonoids, 32 organic acids, 18 monoterpenoids, 15 phenylethanoid glycosides, 13 lignans, 10 triterpenoid saponins, 6 quinones, 5 sugars, 3 alkaloids, and 2 stilbenes.Conclusion In this study, the UHPLC/IM-QTOF-HDMSE method established was suitable to determine and analyze the main chemical composition of Qinggan Yin series preparations, which provides an effective scientific basis for clarifying active substances of Qinggan Yin preparations. |
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