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| Study of total flavonoids from poriacocos on relieving skin pruritus and inhibiting inflammation of psoriasis mice based on IL-17/Notch |
| Hits 498 Download times 340 Received:February 24, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.06.23 |
| Key Words
psoriasis;itchy skin;inflammation;total flavonoids of poriacocos;IL-17/Notch signal axis |
| Author Name | Affiliation | | WANG Xiuju | Department of Dermatology, Zhengzhou Yihe Hospital, Zhengzhou 450047, China | | ZHOU Lijuan | Department of Dermatology, Zhengzhou Central Hospital, Zhengzhou 450000, China | | WANG Kangmin | Department of Dermatology, Zhengzhou Yihe Hospital, Zhengzhou 450047, China | | YU Xiaoxuan | Department of Dermatology, Zhengzhou Yihe Hospital, Zhengzhou 450047, China | | TIAN Zhongwei | Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453100, China |
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| Abstract
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| Objective To investigate the effect of total flavonoids of poriacocos on skin pruritus and inflammation in psoriatic mice, and the regulation of interleukin-17 (IL-17) /Notch signaling axis.Methods The 50 mice were divided into normal group, model group, low-dose group, high-dose group and methotrexate group according to random number table method, with 10 mice in each group. Except the normal group, mice in other groups were depilated on their back and smeared with 5% imiquimod ointment 62.5 mg once a day for consecutive 7 days. Mice in normal group were treated with equal amount of vaseline ointment on their back, mice in low-dose and high-dose groups were treated with 300 and 500 mg/kg total flavonoids of poriacocos, respectively, mice in methotrexate group were treated with 1 mg/kg methotrexate tablet aqueous solution, once a day, for consecutive 7 days. The itching times was evaluated, and PASI score was performed. Serum IFN-γ, IL-23 and tumor necrosis factor-α(TNF-α) levels were detected by ELISA. The pathological lesions of skin tissues were observed by HE staining.Results The epidermis of normal group was thin and only a few inflammatory cells were found. In model group, epidermis thickened, accompanied by hyperkeratosis or parakeratosis, dermal papilla edema, vascular dilation, spinous layer thickened, and a large number of inflammatory cell infiltration can be seen. The pathological damage of mice in low-dose, high-dose and methotrexate groups was reduced to different degrees. Compared with normal group, skin itching times, PASI score, the contents of IFN-γ, IL-23 and TNF-α, the relative expression levels of IL-17, Notch, Jagged1 and HES-1 protein were increased in model group, while the relative expression levels of AQP3 and Cer mRNA were decreased(P < 0.05). Compared with model group, skin itching times, PASI score, contents of IFN-γ, IL-23 and TNF-α, relative expression levels of IL-17, Notch, Jagged1 and Hes-1 were decreased in low-dose, high-dose and methotrexate groups, while relative expression levels of AQP3 and Cer mRNA were increased(P < 0.05). Compared with the low-dose group, the itching times, PASI score, the contents of IFN-γ, IL-23 and TNF-α, the relative expression levels of IL-17, Notch, Jagged1 and Hes-1 were decreased in high-dose group and methotrexate group, while the relative expression levels of AQP3 and Cer mRNA were increased(P < 0.05). Compared with the high-dose group, the itching times, PASI score, the contents of IFN-γ, IL-23 and TNF-α, the relative expression levels of IL-17, Notch, Jagged1 and Hes-1 were decreased in methotrexate group, while the relative expression levels of AQP3 and Cer mRNA were increased (P < 0.05).Conclusion Total flavonoids of poriacocos can improve the skin injury of psoriatic mice, reduce skin pruritus and inhibit the inflammatory response, which may play a regulatory role by inhibiting IL-17/Notch signaling axis. |
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