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| Study on the mechanism of Huazhuo Kangxian Baogan Decoction regulating PI3K/AKT/mTOR pathway to inhibit liver fibrosis |
| Hits 463 Download times 280 Received:March 20, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.07.19 |
| Key Words
liver fibrosis;Huazhuo Kangxian Baogan Decoction;fibroblasts;PI3K/AKT/mTOR |
| Author Name | Affiliation | E-mail | | SUN Ruifang | Department of Gastroenterology, The Second Affiliated Hospital of Xingtai Medical College, Xingtai 054000, China | | | LIU Shizhu | Department of Gastroenterology, The Second Affiliated Hospital of Xingtai Medical College, Xingtai 054000, China | | | LI Ruidong | Radiation and Chemotherapy Division, The Second Affiliated Hospital of Xingtai Medical College, Xingtai 054000, China | | | XU Shuangchao | Department of Gastroenterology, The Second Affiliated Hospital of Xingtai Medical College, Xingtai 054000, China | xushuangchao65@163com |
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| Abstract
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| [Objective] To explore the mechanism of Huazhuo Kangxian Baogan Decoction(HZKXD) regulating PI3K/AKT/mTOR pathway to inhibit liver fibrosis.[Methods] The 80 rats were randomly divided into 4 groups:control group,model group,low-dose HZKXD group and high-dose HZKXD group (n=20). Intraperitoneal injection of carbon tetrachloride (10%) was used to construct a fibrosis model after hepatitis. The intragastric doses of the low-dose HZKXD group and the high-dose HZKXD group were 5 mL/kg and 10 mL/kg,respectively. Peripheral blood liver function indexes,liver tissue damage,liver fibrosis,fibroblast marker protein alpha smooth muscle actin (α-SMA) levels in peripheral blood of rats in each group were compared,as well as PI3K,AKT,mTOR mRNA and protein expressions.[Results] The above indicators of the four groups of rats were significantly different (P<0.05). Model group ALT (127.26±14.03) U/L,AST (260.15±27.57) U/L,liver tissue damage,collagen volume fraction (24.27±1.96)%,α-SMA protein,PI3K,AKT,mTOR mRNA and protein of the control group were significantly higher than those of the control group (P<0.05). ALT (92.85±9.84) U/L,AST (198.67±21.91) U/L,liver tissue damage,collagen volume fraction (14.81±1.04)%,α-SMA protein,PI3K,AKT,mTOR mRNA and protein expressions in the low-dose HZKXD group were significantly lower than those in the model group (P<0.05). ALT (56.44±6.35) U/L,AST (137.23±14.06) U/L,liver tissue damage,collagen volume fraction (7.05±0.85)%,α-SMA protein,PI3K,AKT and mTOR mRNA and protein in the high-dose HZKXD group were significantly lower than those in the model group and low-dose HZKXD group (P<0.05).[Conclusion] HZKXD can alleviate the accumulation of collagen in the liver tissue of liver fibrosis model rats,and can also inhibit the PI3K/AKT/mTOR pathway to reduce fibroblasts. |
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