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| Mechanism of Shexiang Tongxin Dropping Pill in improving cardiac function in diabetic rat with myocardial infarction by promoting angiogenesis |
| Hits 444 Download times 428 Received:February 22, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.08.18 |
| Key Words
Shexiang Tongxin Dropping Pill;diabetes mellitus;myocardial infarction;myocardial microcirculation;angiogenesis |
| Author Name | Affiliation | E-mail | | ZHANG Mengdi | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
Ministry of Education Key Laboratory of traditional Chinese medicine Syndrome and Formula, Beijing 100029, China
BeijingKey Laboratory of traditional Chinese medicine Syndrome and Formula, Beijing 100029, China | | | CUI Xinyu | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
Ministry of Education Key Laboratory of traditional Chinese medicine Syndrome and Formula, Beijing 100029, China
BeijingKey Laboratory of traditional Chinese medicine Syndrome and Formula, Beijing 100029, China | | | YUAN Yueying | Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Yuting | Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | YU Xue | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | WU Yan | Ministry of Education Key Laboratory of traditional Chinese medicine Syndrome and Formula, Beijing 100029, China
BeijingKey Laboratory of traditional Chinese medicine Syndrome and Formula, Beijing 100029, China
Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | 700478@bucm.edu.cn |
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| Abstract
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| [Objective] To explore the protective effect and mechanism of Shexiang Tongxin Dropping Pills on cardiac function in diabetic rats with myocardial infarction from the perspective of promoting angiogenesis. [Methods] Model of diabetic myocardial infarction was established by intraperitoneal injection of streptozotocin (55 mg/kg) and left anterior descending coronary artery ligation. The rats were randomly divided into diabetic myocardial infarction (MI)group and Shexiang Tongxin Dropping Pills (STDP) group [20 mg/ (kg· d)], and a normolglycemic sham operation (Sham) group was established,with 6 rats in each group. Ratsin the STDP group were given Shexiang Tongxin Dropping Pill by gavage every day,while the remaining groups were given the same volume of distilled water. The changes of body weight and fasting blood glucose of rats were observed,and cardiac function was detected by echocardiography after 28 days. HE and Masson staining were used for pathological detection to observe myocardial structure and fibrotic lesions,immunofluorescence wheat Germ Agglutinin (WGA)staining wasusedto measure myocardial cell area. Immunohistochemistry was used to observe myocardial platelet-endothelial cell adhesion molecule-1 (CD31) expression,and the myocardial capillary density was determined. The protein expression levels of VEGF,p-VEGFR2,Ang-1,Tie-2,eNOS,iNOS and p-eNOS in cardiac tissues were determined by Wes automatic Western blot quantitative analysis system. [Results] The diabetic rats showed significant polydipsia,polyphagia and polyuria,and significantly increased blood glucose. Compared with the Sham group,the rats in the MI group showed significantly lower ratios of left ventricular ejection fraction and short-axis shortening,significantly decreased cardiac microvascular density,and significant myocardial fibrosis and cardiomyocyte hypertrophy (P<0.01). Meanwhile,VEGF,p-VEGFR2,Ang-1,Tie -2,eNOS and p-eNOS protein expression were significantly decreased (P<0.01) and iNOS expression level was significantly increased (P<0.01). Compared with the MI group, myocardial fibrosis and cardiomyocyte hypertrophy were significantly improved,microvessel density was increased,protein expression levels of VEGF,p-VEGFR2,Ang-1,Tie-2,eNOS and p-eNOS were increased (P<0.01 or P<0.05),and iNOS protein expression was decreased (P<0.05) in the STDP group rats,and cardiac function was significantly improved. [Conclusion] Shexiang Tongxin Dropping Pills could improve the cardiac function of diabetic rats with myocardial infarction,which was associated with promoting angiogenesis and improving myocardial microcirculation disorders. |
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