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| Effects of alcohol extract of Xiefei Lishui Formula on heart function in zebrafish model of heart failure with different administration routes |
| Hits 477 Download times 249 Received:March 15, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.08.19 |
| Key Words
heart failure;alcohol extract of Xiefei Lishui Formula;different administration routes;zebrafish;heart function |
| Author Name | Affiliation | E-mail | | GONG Tao | Beijing Institute of Traditional Chinese Medicine, Beijing 100035, China | | | TONG Tong | Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China | | | ZHOU Deyong | Beijing Institute of Traditional Chinese Medicine, Beijing 100035, China | | | YAN Wenli | Beijing Institute of Traditional Chinese Medicine, Beijing 100035, China | | | XIA Bo | Hunter Biotechnology, Inc., Hangzhou 310051, China | | | YANG Yiwen | Hunter Biotechnology, Inc., Hangzhou 310051, China | | | CAO Huizhuo | Hunter Biotechnology, Inc., Hangzhou 310051, China | | | LIU Hongxu | Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China | lhx_@263.net |
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| Abstract
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| [Objective] To explore the effects of Alcohol Extract of Xiefei Lishui Formula on heart failure with different administration routes. [Methods] Verapamil induced heart failure model in zebrafish was used to evaluate the improvement of cardiac function by injection and water-soluble administration,the intervention dose and concentration were determined according to the maximum dose and concentration of alcohol extract from Xiefei Lishui Formula,and the improvement of heart failure was evaluated by measuring the heart area,venous congestion area,blood flow velocity and cardiac output. [Results] The maximum does and maximum concentration of alcohol extracted Xiefei Lishui Formula were 100 ng/tail and 500 μg/mL；injection had significant improvement on heart area,venous congestion area, blood flow velocity and cardiac output of zebrafish,the improvement rates of low,medium,high dose group were 33%,41%,34%； 44%,57%,56%；33%,48%,58%；28%,38%,72%；water-soluble administration did not have evident improvement on heart area and venous congestion area of zebrafish,the improvement rates of low,medium,high dose group were -5%,7%,28% and -18%,-6%,-1%, but could improve the blood flow velocity and cardiac output obviously,the improvement rates of low,medium,high dose group were 23%,24%,28% and 22%,22%,31%. [Conclusion] The injection of alcohol extracted Xiefei Lishui Formula can significantly improve the cardiac function in zebrafish model of verapamil-induced heart failure. |
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