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| Exploration of the protective effects of Leech and Earthworm extracts on cerebral ischemic penumbra neurons in MCAO/R mice based on PI3K/AKT pathway |
| Hits 442 Download times 495 Received:February 03, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.08.20 |
| Key Words
Leech;Earthworm;cerebral ischemia/reperfusion injury;PI3K/AKT signaling pathway;apoptosis |
| Author Name | Affiliation | E-mail | | ZHAO Lei | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministy of Education, Tianjin 301617, China | | | YUAN Qing | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministy of Education, Tianjin 301617, China | | | ZHANG Tong | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministy of Education, Tianjin 301617, China | | | JIA Zhuangzhuang | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministy of Education, Tianjin 301617, China | | | YIN Menglan | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministy of Education, Tianjin 301617, China | | | HU Limin | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministy of Education, Tianjin 301617, China | hulimin@tjutcm.edu.cn |
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| Abstract
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| [Objective] To explore the protective effect and mechanism of leeches and earthworm extracts on cerebral ischemic penumbra neurons in MCAO/R mice. [Methods] Forty 8-week-old healthy C57BL/cnc male mice were selected to prepare cerebral ischemia- reperfusion mouse models using the middle cerebral artery suture method. After successful modeling,they were divided into sham operation group (Sham) and brain by random number table method. Ischemia-reperfusion group (MCAO/R),leech extract group,earthworm extract group. Each administration group was administered by tail vein injection once a day for 7 consecutive days. mNSS score to evaluate the neurological damage of mice,HE staining to detect the pathological changes in the cerebral ischemic penumbra of each group of mice,TUNEL staining to detect the cell apoptosis rate in the cerebral ischemic penumbra of mice,Nissl staining to observe the degree of neuronal damage,Western Blot method to detect B lymphoma-2 (Bcl-2),Bcl-2 related X protein (Bax),activated Caspase-3 (Cleaved Caspase-3),Caspase-3,PI3K,AKT,p-AKT,neuron nuclear protein (NeuN) and microtubule-associated protein 2 (MAP2) protein expression,immunofluorescence detection of Bax,Bcl-2 expression. [Results] Compared with the MCAO/R group,the neurological deficit,tissue morphology,and neuron damage in the leech extract group and ground dragon extract group were improved to varying degrees,and the cerebral ischemic penumbra PI3K and Bcl-2 were up-regulated,NeuN,MAP2 protein expression and increase the ratio of p-AKT/AKT and Bcl-2/Bax. [Conclusion] The extracts of leech and earthworm can ameliorate neuronal damage in the cerebral ischemic penumbra of MCAO/R mice,and the mechanism may be through activation of the PI3K/AKT signaling pathway. |
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