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| Effect of geniposide on TGF-β/Smad pathway in high glucose-induced trans-differentiation of renal tubular epithelial cells |
| Hits 409 Download times 345 Received:March 12, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.08.21 |
| Key Words
geniposide;renal tubular epithelial cell;trans-differentiation;transforming growth factor-β and Smad related protein pathway |
| Author Name | Affiliation | E-mail | | LIANG Dong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | YANG Hongtao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | tjtcmht@126.com |
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| Abstract
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| [Objective] To investigate the effects of geniposide (GE) on transforming growth factor-β and Smad related protein (TGF-β/ Smad) pathway in high glucose-induced trans-differentiation of renal tubular epithelial cells. [Methods] The experiment was divided into control group,high glucose group and GE 1,10,50,100 μmol/L groups. HK2 cells in the control group were cultured in 5.5 mmol/L low glucose DMEM medium. Except the control group,the other groups were cultured in 25 mmol/l high glucose DMEM medium,GE 1 ,10,50,100 μmol/L groups were added with GE at the same time,and the final concentration of GE was 1,10,50, 100 μmol/L respectively for 48 h. The morphology of hK2 cells was observed under microscope,the mRNA levels of fibronectin (FN),E-cadherin (E-cad) and collagen type Ⅳ (COLⅣ) were detected by real-time fluorescence quantitative PCR (qRT-PCR),the levels of interleukin (IL)-1β,IL-6 and tumor necrosis factor-α (TNF-α) in the supernatant were detected by enzyme-linked immunosorbent assay (ELISA), the protein levels of TGF-β1,Smad3,and α-smooth muscle actin (α-SMA) were detected by Western blot. [Results] The cells in the control group were polygonal or oval in shape and evenly distributed；in the high glucose group,some of the cells were spindle shaped and the nucleus was spindle shaped,with the increase of GE dose,the cell morphology gradually returned to normal. Compared with those in the control group,the mRNA levels of FN and COLⅣ,the protein levels of TGF-β1,p-Smad3/Smad3,α-SMA protein,and the levels of IL-1β,IL-6 and TNF-α in the supernatant in high glucose group were higher,and the mRNA level of E-cad was lower (P<0.05). Compared with those in the high glucose group,the mRNA level of FN,the protein levels of TGF-β1,p-Smad3/Smad3,α-SMA protein,and the levels of IL-1β,IL-6 and TNF-α in the supernatant in GE 1 μmol/L group were lower (P<0.05),in GE (10,50,100) μmol/L group, the mRNA levels of FN and COLⅣ,the protein levels of TGF-β1,p-Smad3/Smad3,α-SMA protein,and the levels of IL-1β,IL-6 and TNF-α in the supernatant were lower,and the mRNA level of E-cad in the cells was higher (P<0.05). [Conclusion] GE can inhibit TGF- β/Smad pathway in high glucose-induced trans-differentiation of renal tubular epithelial cells. |
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