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| Effects of Jiangtang Xiaoke Granule on hepatic lipid metabolism and endoplasmic reticulum stress in diabetic mice |
| Hits 601 Download times 366 Received:March 25, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.09.16 |
| Key Words
type 2 diabetes;non-alcoholic fatty live disease;Jiangtang Xiaoke Granule;hepatic lipid metabolism;endoplasmic reticulum stress |
| Author Name | Affiliation | E-mail | | ZHANG Yi | Beijing Open University, Beijing 100081, China | | | ZHAO Dandan | Beijing University of Chinese Medicine, Beijing 100029, China | | | MO Fangfang | Beijing University of Chinese Medicine, Beijing 100029, China | | | GAO Sihua | Beijing University of Chinese Medicine, Beijing 100029, China | gaosihua1216@163.com |
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| Abstract
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| [Objective] To investigate the effect of Jiangtang Xiaoke Granule (JTXKG) on correlative indexes of endoplasmic reticulum stress and lipid metabolism in type 2 diabetic KK-Ay mice which were induced by high fat diet.The effect of JTXKG on T2DM was discussed from releasing the hepatic lipid metabolism disorders.[Methods] Male 8-week-old KK-Ay mice,were fed by high-fat-diet,T2DM model were established by FBG ≥ 13.9 mmol/L.The T2DM mice were then randomly divided into model group,pioglitazone group and JTXKG groups in low (1.75 g/kg),medium (3.5 g/kg),and high (7 g/kg) doses.The normal group had C57BL/6J in it and feed by normal diet.After 10-week treatment,tissue from the same part of the liver were taken and fixed with 10% formalin,the rest of the liver tissue was stored in liquid nitrogen.the liver gene expression of IRE1α,XBP1,SREBP-1c,SREBP2,Insig-1,FAS were examined by real-time fluorescence quantitative PCR (FQ-PCR),the protein expression of p-eIF2α and GRP78 were examined by immunohistochemistry method and the effects of JTXKG on the above indexes were observed.[Results] In this experiment,the gene expression of SREBP-1c,SREBP2,IRE1α,XBP1 increased significantly (P<0.01),respectively,the gene expression of Insig-1in model group decreased significantly (P<0.01) and the gene expression of FAS increased (P<0.05).T2DM mice supplemented with 7 g/kg dose of JTXKG have lower gene expression of SREBP-1c (P<0.01).Both 3.5 g/kg and 1.75 g/kg dose of JTXKG have a significant effect on reducing the gene expression of SREBP-1c,SREBP2(P<0.01) and FAS (P<0.05).Otherwise,3.5 g/kg dose of JTXKG has increased the gene expression of IRE1α and decreased the gene expression of Insig-1(P<0.05).All dose of JTXKG could reduce the protein expression of p-eIF2α and GRP78 significantly (P<0.01).[Conclusion] Hepatic lipid synthesis of T2DM mice is correlated with endoplasmic reticulum stress.Both 3.5 g/kg and 1.75 g/kg dose of JTXKG can decrease hepatic lipogenesis,reduce lipid deposition and inhibit the endoplasmic reticulum stress in the liver of T2DM mice.In this way,the lipid metabolism disorder of T2DM mice can be relieved. |
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