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| The effect of Xifeng Capsule combined with carbamazepine on hippocampal voltage-gated sodium channel of epilepsy rat models indused by Lithium-pilocarpine |
| Hits 576 Download times 317 Received:December 03, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.09.18 |
| Key Words
voltage-gated sodium channel;Xifeng Capsule;carbamazepin;epilepsy |
| Author Name | Affiliation | E-mail | | LU Yanli | Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | FANG Yanyan | Pediatrics, Chinese Medicine Hospita in Linyi, Linyi 276002, China | | | LI Xinmin | Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | lixinmin1998@sohu.com | | SUN Dan | Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | HAN Yaowei | Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | CHEN Zumin | Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | |
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| Abstract
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| [Objective] To observe the effects of Xifeng Capsule combined with carbamazepine (CBZ) on voltage-gated sodium channels (VGSC) in hippocampal neurons of epileptic SD rats induced by lithium chlorine-pilocapine.[Methods] The 20 SD rats were randomly selected from 110 SD rats as blank control group,and 80 rats were randomly selected after successful modeling,which were divided into model control group,Xifeng Capsule treatment group,Xifeng Capsule combined with CBZ treatment group,and CBZ treatment group.The expression of VGSC was detected by immunohistochemistry and its electrophysiological function was detected by whole cell patch clamp.[Results] 1) Recurrent spontaneous seizures occurred in the epileptic rats,and the frequency of seizures in the Xifeng Capsule combined with CBZ group was lower than that in the Xifeng Capsule treatment group and the CBZ group.2) Expression of VGSC:the expression level of VGSC in all treatment groups was down-regulated in CA3 region compared with that in model control group,and the expression level of VGSC in Xifeng Capsule combined with CBZ group was significantly lower than that in wind quenching group and CBZ group.3) Electrophysiological function of VGSC:compared with the model control group,the peak current in the treatment group decreased significantly,especially in the card extinguishing group compared with the Xifeng Capsule treatment group and CBZ group.The half steady-state activation voltage in the treatment group increased,but there was no difference between the groups.The 50% steady-state inactivation voltage of the treatment group decreased,and there was a difference between the Xifeng Capsule combined with CBZ group and the Xifeng Capsule treatment group.After inactivation,the recovery time constant of the treatment group was significantly prolonged,and there was a difference between the Xifeng Capsule combined with CBZ group and the Xifeng Capsule treatment group.[Conclusion] Xifeng Capsule and CBZ have synergistic antiepileptic effect.The main pharmacological mechanism superior to monotherapy is to reduce peak VGSC current. |
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