|
| Breviscapine regulates the effect of Nrf2/Gpx4 pathway on myocardial apoptosis in MIRI model and its mechanism |
| Hits 446 Download times 273 Received:March 09, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2022.09.20 |
| Key Words
myocardial ischemia-reperfusion injury;breviscapine;nuclear factor E2-related factor 2/Glutathione peroxidase 4;apoptosis;oxidative stress |
| Author Name | Affiliation | | SHI Lulu | Zhangjiakou First Hospital, Zhangjiakou 075000, China | | HAN Weinan | Zhangjiakou First Hospital, Zhangjiakou 075000, China | | WANG Qiang | Zhangjiakou First Hospital, Zhangjiakou 075000, China | | XU Ningning | Zhangjiakou First Hospital, Zhangjiakou 075000, China |
|
| Abstract
|
| [Objective] To explore the effect and mechanism of breviscapine's regulation of nuclear factor E2-related factor 2(Nrf2)/glutathione peroxidase 4(Gpx4) pathway on myocardial apoptosis in myocardial ischemia reperfusion injury (MIRI) models.[Methods] The 45 rats were randomly divided into Sham group,MIRI group and MIRI+breviscapine group (n=15).The MIRI model was established by ligating the left anterior descending coronary artery.After 24 h of modeling,the MIRI+breviscapine group was given breviscapine by gavage (200 mg/kg,1 time/d,7 d).Cardiac function,myocardial tissue injury,apoptosis,serum oxidative stress factor,and Nrf2/Gpx4 pathway expression levels were compared in each group.[Results] The above indicators of the three groups were significantly different (P<0.05).LVESP,±dP/dt max,SOD,Nrf2 and Gpx4 mRNA in MIRI group.And protein levels were significantly lower than those in Sham group,LVEDP,apoptosis index,MDA levels were significantly higher than thosein Sham group (P<0.05).LVESP,±dP/dt max,SOD,Nrf2 and Gpx4 mRNA and protein levels in MIRI+breviscapine group were significantly higher than those in MIRI group,while LVEDP,apoptosis index and MDA were significantly lower than those in MIRI group (P<0.05).[Conclusion] Breviscapine has the function of alleviating myocardial cell apoptosis in MIRI model rats,and its mechanism of protecting heart function may be related to the promotion of Nrf2/Gpx4 pathway. |
|
|
|
|
|
|
|
