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| Study on the mechanism of Shufeng Zhijing Prescription in the treatment of drug-resistant epilepsy based on network pharmacology and molecular docking |
| Hits 428 Download times 346 Received:June 10, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.09.21 |
| Key Words
network pharmacology;Shufeng Zhijing Prescription;drug-resistant epilepsy;action mechanism;molecular docking |
| Author Name | Affiliation | E-mail | | CUI Fen | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | RONG Ping | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | WANG Lin | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | wltjzy@sina.cn |
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| Abstract
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| [Objective] To explore the mechanism of Shufeng Zhijing Prescription in the treatment of drug-resistant epilepsy.[Methods] The 12 active components of Shufeng Zhijing Prescription were screened from the pharmacological database (TCMSP) of traditional Chinese medicine system,and the action targets were predicted;drug-resistant epilepsy-related genes were obtained by GeneCards and OMIM databases,and DRE-related targets were obtained.The intersection targets of drugs and diseases were screened by R4.0.0 language,and the target protein interaction network was constructed with the help of String database.The traditional Chinese medicine-chemical composition-disease target network was constructed by Cytoscape3.7.2 software;the enrichment analysis of GO and KEGG was carried out by R language,and the KEGG relationship network was constructed by Cytoscape software.Finally,the molecular docking of important compounds was verified by AutoDockVina.[Results] Finally,165 potential targets and quercetin,luteolin,kaempferol,wogonin and naringin were screened out.2 584 GO functional items and 171 KEGG pathways were identified by gene enrichment analysis of 7 core targets:PTGS2,HSP90AA1,ESR1,AR,MAPK1,AKT1 and MAPK3.The results of molecular docking showed that the main active components of Shufeng Zhijing Prescription had good binding activity to the core targets.[Conclusion] This study reveals the mechanism of Shufeng Zhijing Prescription in the treatment of drug-resistant epilepsy,which mainly plays a synergistic effect through antiviral,anti-inflammation and neuroprotection,which provides a theoretical basis for rational clinical application. |
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