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| Effect of "astragalus-salvia" on renal interstitial fibrosis and hypoxia inducible Factor-1α expression in rats with unilateral ureteral obstruction |
| Hits 486 Download times 297 Received:June 10, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.10.16 |
| Key Words
renal interstitial fibrosis;astragalus-salvia;UUO;HIF-1α |
| Author Name | Affiliation | E-mail | | YANG Xikai | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | 王耀光，wangyaoguang1012@126.com | | WANG Yaoguang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | |
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| Abstract
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| [Objective] To explore the effects of "astragalus-salvia" on renal interstitial fibrosis and renal tubular epithelial cell transdifferentiation (EMT) and the expression of HIF-1α through the unilateral ureteral obstruction (UUO) model.[Methods] The rats were divided into sham operation group,model group,low-dose Chinese medicine group,middle-dose Chinese medicine group,high-dose Chinese medicine group,and Losartan group.The UUO model was used and the stomach was given and the materials were taken.Observe rat Masson staining;Western Blot method to detect HIF-1α protein expression;immunohistochemical method to detect rat α-SMA protein expression;PCR to detect rat Col1 and FN mRNA expression.[Results] Masson staining:compared with the model group and the sham operation group,the collagen volume was significantly increased (P<0.001);the medium and high doses of Chinese medicine decreased compared with the model group (P<0.01);Losartan group was significantly lower than model group (P<0.001).Immunohistochemistry:compared with the sham operation group,the expression of α-SMA protein increased significantly in the rat model group (P<0.001);compared with the model group in the middle and high doses of Chinese medicine and the losartan group,α-SMA decreased significantly (P<0.01).Real-time fluorescence quantitative PCR detection:compared with the sham operation group,the expression levels of Col1 and FN increased significantly in the model group (P<0.001),and the expression levels of Col1 and FN decreased in the drug group compared with the model group (P<0.05).Western Blot detection:compared with the sham operation group,the expression of HIF-1α was significantly increased in the model group (P<0.001);the dose of Chinese medicine was significantly lower than that in the model group (P<0.01).[Conclusion] "Astragalus-salvia" can delay renal interstitial fibrosis by inhibiting the production of extracellular matrix and reducing the transdifferentiation of renal tubular epithelial cells-mesenchymal cells.The mechanism may be related to the inhibition of HIF-1α expression. |
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