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| Study on the effect and mechanism of Qinggan Qiuyin Formula in the prevention of pharyngitis based on network pharmacology |
| Hits 747 Download times 387 Received:July 28, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.10.17 |
| Key Words
pharyngitis;inflammation;Qinggan Qiuyin Formula;mononuclear macrophage;network pharmacology |
| Author Name | Affiliation | E-mail | | LU Jia | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617 China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formula of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | 苗琳，mmmlin@tjutcm.cn;张晗，zhanghan0023@126.com | | SU Rui | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617 China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formula of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YAN Huimin | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617 China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formula of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YAN Yiqi | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617 China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formula of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MIAO Lin | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617 China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formula of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Han | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617 China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formula of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| [Objective] To investigate the effect and mechanism of Qinggan Qiuyin (QGQY) for the prevention and treatment of pharyngitis.[Method]For the network pharmacological research,active ingredients in QGQY was collected through screening from the traditional chinese medicine systems pharmacology database and analysis platform (TCMSP) and symptom mapping database (SymMap),and target information of the active ingredients from QGQY were then obtained from TCMSP database.Pharyngitis related targets were obtained through the Human Gene database (GeneCards) and a database of gene-disease associations (DisGeNET).The intersection of QGQY and pharyngitis targets was taken to construct Protein-Protein Interaction networks (PPI),and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis were further performed to figure out the related targets by the database for annotation visualization and integrated discovery (DAVID).For experimental assays,lipopolysaccharide (LPS)-stimulated THP-1 cell and RAW264.7 macrophages were used as cellular models for inflammation,and the effects of QGQY on the cytokine expressions in LPS-induced THP-1 cells was measured by RT-PCR.NO level in RAW 264.7 cells was detected by ELISA.Nuclear factor kappa-B (NF-κB) promoter activity was evaluated by the dual luciferase reporter system.[Results] By network pharmacological screening,191 active components and 276 potential predictive targets of QGQY were obtained and 562 disease targets of upper respiratory tract infection were set,which were further overlapped and 59 common targets were finally confirmed.PPI networks were then constructed and key targets such as signal transducer and activator of transcription 3(STAT3),Tumor necrosis factor (TNF),Interleukin-6(IL-6),Interleukin-1β(IL-1β),Interleukin-10(IL-10) and Interferon-inducible protein-10(IP-10) were obtained through topology and cluster analysis.Functional enrichment analysis included a total of 372 GO items involving biological processes,molecular functions and cellular components,which were mainly involved in the drug response,apoptosis,extracellular space,enzyme binding and cytokine activity.KEGG enrichment result showed that QGQY regulated respiratory infectious through 85 signaling pathways,including TNF,Hypoxia inducible factor-1(HIF-1),T cell receptor,NF-κB and other inflammatory signaling pathways.QGQY significantly reduced the expressions of cytokines TNF-α,IL-1β,IL-6,NF-kappa-B inhibitor alph (IKBα) and IP-10 in LPS-induced THP-1 cells and the content of NO in LPS-induced RAW264.7 cells,while inhibiting the activity of NF-κB promoter induced by TNF-α.[Conclusion] QGQY has potential anti-inflammatory effects,and it may work together through multiple components,targets and pathways to inhibit the occurrence and development of pharyngitis. |
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