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| Study on the effect of puerarin on alleviating high glucose-induced hypertrophy of H9c2 cells via activating AMPK/Akt/GSK-3β signaling pathway |
| Hits 496 Download times 244 Received:July 15, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.10.18 |
| Key Words
puerarin;diabetic cardiomyopathy;high glucose;AMPK/Akt/GSK-3β signaling pathway;myocardial hypertrophy |
| Author Name | Affiliation | E-mail | | XU Hong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | 李玉红，liyuhong@tjutcm.edu.cn | | LI Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China | | | PAN Guixiang | Second Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | LI Yuhong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China | |
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| Abstract
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| [Objective] The aim of the present study was to examine the effects of puerarin on H9c2 cells injured by high glucose.[Methods] H9c2 cells were injured by high glucose for 48 h,and diabetic myocardial injury cell model was established in vitro.The cytoskeleton was stained with rhodamine phalloidin,and the cell surface area was determined.Western blot was used to detect the expression of β-MHC,phosphorylation of AMPKα,Akt and GSK-3β,and protein levels of IP3R2 and SIG-1R.[Results] Puerarin (20 μmol/L) could significantly inhibit the increase of cardiomyocyte surface area induced by high glucose.Western blot analysis showed that high glucose up-regulated β-MHC protein levels and inhibited phosphorylation of AMPK and Akt,while puerarin down-regulated β-MHC and IP3R2 expression and promoted phosphorylation of AMPK,Akt and GSK-3β.[Conclusion] Puerarin may alleviate hypertrophy of H9C2 cells induced by high glucose via activating AMPK/Akt/GSK-3β signaling pathway. |
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