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| Mechanistic analysis of Sagacious Confucius'Pillow Elixir in the treatment of insomnia and amnesia with concept of "treating different diseases with same method" based on network pharmacology |
| Hits 503 Download times 323 Received:August 10, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.10.19 |
| Key Words
network pharmacology;Sagacious Confucius'Pillow Elixir;insomnia;amnesia;treating different diseases with same method |
| Author Name | Affiliation | E-mail | | CHEN Jing | Basic Medical School, Heilongjiang University of Chinese Medicine, Harbin 150040, China | 侯志涛，18745020244@139.com | | YUAN Min | Basic Medical School, Heilongjiang University of Chinese Medicine, Harbin 150040, China | | | HOU Zhitao | Basic Medical School, Heilongjiang University of Chinese Medicine, Harbin 150040, China
Key Laboratory of Chinese Internal Medicine of Ministry of Education, Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China | |
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| Abstract
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| [Objective] To explore the possible mechanism of Sagacious Confucius'Pillow Elixir in the treatment of insomnia and amnesia with concept of "treating different diseases with same method" by the network pharmacology.[Methods] Using TCMSP,HIT 2.0,TCMIP,natural Product Chemistry Database of China and other network platforms,to screen the active ingredients of Sagacious Confucius'Pillow Elixir (SCPE) formula.The targets of its active ingredients were extracted from Uniprot protein database (Uniprot),and the targets related to insomnia and amnesia were extracted from the Comparative Toxicological Genomics Database (CTD).After comparing and screening the targets of drug active components and disease targets,Cytoscape 3.7.1 software was used to construct a "drug-component-target-disease" network model for SCPE treatment of insomnia and amnesia.Protein interaction (PPI) networks were mapped using the Protein Interaction Database (String) to find key targets.Biological process data analysis using annotation,Visualization and Integrated Discovery Database (DAVID),Gene Annotation and Analysis Database (Metascape),R Studio software and other platforms,the results of Heatmap analysis,Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were obtained.[Results] We obtained 39 active components of SCPE,24 771 targets for insomnia and 55 876 targets for amnesia,83 targets of SCPE for insomnia and amnesia.Biological process enrichment analysis of the top 20 signal pathway of SCPE for insomnia and amnesia include tumor necrosis factor (TNF) signal pathway,vascular endothelial growth factor (VEGF) signal pathway,p53 signal pathway,CPMP-PKG signal pathway,etc.The main mechanisms of insomnia and amnesia of SCPE may involve anti-apoptosis,inflammatory response,neurotransmitter regulation,ubiquitin protein ligase binding,etc.[Conclusion] Insomnia and amnesia involve multiple components,multiple targets and multiple pathways,which provides a new idea and way to explain the scientific connotation of TCM compound "treating different diseases with the same treatment" and also provide bioinformatics theoretical basis for further mechanism research and new clinical application of SCPE. |
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