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| Mechanism study of Mahuang Fuzi Xixin Decoction combined with Fangji Huangqi Decoction on reducing oxidative stress injury in mouse with membranous nephropathy |
| Hits 424 Download times 787 Received:September 08, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.11.15 |
| Key Words
Mahuang Fuzi Xixin Decoction;Fangji Huangqi Decoction;membranous nephropathy |
| Author Name | Affiliation | E-mail | | HUANG Shan | Department of Traditional Chinese Medicine, Tianjin First Central Hospital, Tianjin 300192, China | | | LIU Nawen | Department of Traditional Chinese Medicine, Tianjin First Central Hospital, Tianjin 300192, China | nn1747@sina.com | | HAO Yang | Department of Traditional Chinese Medicine, Tianjin First Central Hospital, Tianjin 300192, China | |
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| Abstract
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| [Objective] To observe the effectiveness of Chinese medicine Mahuang Fuzi Xixin Decoction combined with Fangji Huangqi Decoction in treating mouse model of membranous nephropathy prepared using cationized bovine serum albumin (C-BSA).And its effect on the expression of SOD,MDA,IL-6,MCP-1 and NF-κB in renal tissue.[Methods] The 60 BALB/C mice were randomly divided into normal group,model group,low,middle and high dose group,12 in each.The mouse model of membranous nephropathy was established by C-BSA. Take the serum six weeks after administration.The levels of ALB,TG,TC,SCR and Bun in mice were detected by automatic biochemical analyzer. HE staining and PAS staining were used to observe the morphological changes of renal tissue. The expression of SOD,MDA,IL-6 and MCP-1 in kidney was detected by ELISA system. The expression of NF-κB protein was detected by immunohistochemical staining.[Results] After six weeks of medication,compared with the model group,the levels of TG and TC in low,middle and high dose groups decreased in different degrees (P<0.05 or P<0.01),the levels of ALB are significantly elevated (P<0.01).The structure of the glomerulus and renal tubules of the mice were improved to some extent compared with the model group. The content of SOD in kidney tissue increased in different degree (P<0.05 or P<0.01).The contents of MDA,IL-6 and MCP-1 decreased significantly (P<0.01). The expression of NF-κB protein in the kidney of mice decreased in different degree (P<0.05 or P< 0.01).[Conclusion] Mahuang Fuzi Xixin Decoction combined with Fangji Huangqi Decoction can effectively improve lipid metabolism disorder,increase plasma albumin level,alleviate renal pathological damage in mice with membranous nephropathy,and have therapeutic effect on mice with membranous nephropathy.It can effectively inhibit the oxidative stress and inflammation in the kidney of mice with membranous nephropathy. The mechanism may be related to the inhibition of NF-κB pathway. |
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