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| Stigmasterol inhibits the contraction of rat prostatic stromal cells by inhibiting calcium influx |
| Hits 428 Download times 502 Received:August 03, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.11.16 |
| Key Words
stigmasterol;prostatic stromal contraction;benign prostatic hyperplasia;α-smooth muscle actin;calcium |
| Author Name | Affiliation | E-mail | | MA Yaxuan | State Key Laboratory of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Key Laboratory of Formulary Science, Ministry of Education, Tianjin 301617, China | | | WU Yaoxin | State Key Laboratory of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Key Laboratory of Formulary Science, Ministry of Education, Tianjin 301617, China | | | SHAO Rui | State Key Laboratory of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Key Laboratory of Formulary Science, Ministry of Education, Tianjin 301617, China | rizycarrot@126.com | | MIAO Lin | State Key Laboratory of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Key Laboratory of Formulary Science, Ministry of Education, Tianjin 301617, China | mmmlin@tjutcm.cn |
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| Abstract
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| [Objective] To detect the inhibitory effect of stigmasterol on the contraction of prostate stromal cells.[Method] Rat primary prostatic stromal cells were cultured in vitro and the phenotypes were identified by phalloidin staining. The effects of stigmasterol on cell viability and toxicity were investigated by CCK8 and LDH assays. The contractile response of prostate stromal cells was analyzed by collagen gel assay. RT-PCR and Western Blot were performed to detect the basal and transforming growth factor-β1 (TGF-β1) induced expressions of α-smooth muscle actin(α-SMA) and CollagenⅠ. Acetylcholine(ACh) induced intracellular calcium levels was established and the effect of stigmasterol on ACh-induced Ca2+ concentration was further detected by flow cytometry.[Results] Cells obtained from the rat prostate were mainly identified as the interstitial smooth muscle cells;stigmasterol had no significant effects on the viability and toxicity of prostatic stromal cellswith concentrations lower than 10μM;collagen gel assay showed that seruminducedthe contraction of the prostatic stromal cells (P<0.01),which was significantly inhibited bystigmasterol,andthe inhibition rate reached 77.1% at 10 μmol/L (P<0.05). RT-PCR data showed that stigmasterol significantly down-regulated the mRNA expressions of α-SMA and CollagenⅠ in rat prostatic stromal cells(P<0.01). Western Blot also showed that stigmasterol inhibited TGF-β1 induced α-SMA expression. The increased calcium levelin response to ACh stimulation was also significantly inhibited by stigmasterol.[Conclusion] Stigmasterol inhibits the contraction of prostate stroma through inhibition of calcium influx. |
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