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| Impact of galangin on myocardial injury in diabetic rat by inhibiting IRAK-1/MAPK/NF-κB signaling pathway |
| Hits 372 Download times 431 Received:July 28, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.11.20 |
| Key Words
galangin;interleukin-1 receptor-associated kinase-1;mitogen-activated protein kinase;nuclear factor-κB signaling pathway;diabetes;rat;myocardial injury |
| Author Name | Affiliation | E-mail | | LYU Chaoyang | Department of Endocrinology, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | | | YANG Yingjun | Department of Endocrinology, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | yingjun-yang@163.com | | AO Wen | Department of Endocrinology, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | | | LI Zhenzhen | Department of Endocrinology, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | | | HUANG Ting | Department of Endocrinology, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | | | XU Zaige | Department of Endocrinology, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | | | LIU Huishuang | Department of Nutrition, The 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China | |
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| Abstract
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| [Objective] To investigate whether galangin can alleviate myocardial injury in diabetic rats by inhibiting the interleukin-1 receptor-associated kinase-1 (IRAK-1)/mitogen-activated protein kinase (MAPK)/nuclear factor-κB (NF-κB) signaling pathway.[Methods] After continuous feeding with high sugar and high fat for 8 weeks,30 mg/kg streptozotocin (STZ) was injected intraperitoneally at one time to establish a model,the successful diabetic model rats were randomly grouped into model group,galangin group,galangin+ pc-NC group,and galangin+pc-IRAK-1 group,8 animals per group.The 8 rats in the control group were fed normally for the same time. Glucose meter was applied to measure blood sugar level;cardiacultrasound was used to detect left ventricular end-diastolic diameter (LVEDD),left ventricular end-systolic diameter (LVESD) and left ventricular ejection fraction (LVEF);masson staining was applied to detect myocardial tissue fibrosis and to analyze myocardial collagen volume percentage(CVF);transmission electron microscopy was applied to observe the ultrastructure of myocardial tissue;kits were applied to detectthe levels ofmalondialdehyde (MDA),superoxide dismutase (SOD),lactate dehydrogenase (LDH),creatine kinase (CK),nitric oxide (NO),and nitric oxide synthase (NOS);western blot was used to detect the protein expression of inducible nitric oxide synthase (iNOS),endothelial nitric oxide synthase (eNOS), IRAK-1,p38 MAPK,and NF-κB p65 in myocardial tissue.[Results] After modeling and before administration,the blood glucose level increased (P<0.05),the molding successed.After administration,the interstitial and paravascular myocardial cells of the model group were stained deeply,and the myocardial structure was disordered under the transmission electron microscope,and some myofilaments were dissolved;the above symptoms in the galangin group were relieved,and the symptoms in the galangin +pc-IRAK-1 group were between the model group and the galangin group.Compared with the control group,the blood glucose,LVEDD,LVESD and CVF levels, MDA content,iNOS,IRAK-1,p38 MAPK and NF-κB p65 protein levels in the model group increased (P<0.05),the LVEF level,CK and NO contents,SOD,LDH and NOS activitiesand eNOS protein level decreased(P<0.05);compared with the model group,the blood glucose,LVEDD,LVESD and CVF levels,MDA content,iNOS,IRAK-1,p38 MAPK and NF-κB p65 protein levels in the galangin group decreased (P<0.05),the LVEF level,CK and NO contents,SOD,LDH and NOS activities and eNOS protein levelincreased (P<0.05); elevating the expression of IRAK-1 on the basis of galangin treatment could inhibit the effect of galangin.[Conclusion] Galangin can alleviate myocardial injury in diabetic rats by inhibiting IRAK-1/MAPK/NF-κB signaling pathway. |
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