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| Intervention of Chinese herbs for stasis removing and collaterals dredging on podocyte density in the diabetic nephropathy rats |
| Hits 962 Download times 795 Received:August 26, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.12.15 |
| Key Words
diabetic nephropathy;Chinese herbs for stasis removing and collaterals dredging;proteinuria;integrin α3β1 |
| Author Name | Affiliation | E-mail | | BAI Lu | Department of Nephrology, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, China | | | CHEN Zhiqiang | Second Department of Nephrology, First Affiliated Hospital of Hebei University of Traditional Chinese Medicine, Shijiazhuang 050011, China | chenzhqiang2011@163.com |
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| Abstract
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| [Objective] To discuss the effect of Chinese herbs for stasis removing and collaterals dredging upon the the expression of renal integrin α3β1 and podocyte density in the renal of diabetic nephropathy rats. [Methods] The diabetes rat model was induced by feeding high-glucose high-fat diet combination with intraperitoneal injection of STZ. Rats were divided into normal group,model group,Chinese medicine group,and irbesartan group,all groups were given relevant medicinal intragastrically. Lavage for 20 weeks,detected rats' fasting blood glucose (FBG),serum creatinine (Scr),urea nitrogen (BUN),24h urinary protein quantitative (24h UTP). Renal tissue was examined by light microscopy. The expression of renal ITGA3,ITGB1,WT1 mRNA and protein were determined by Real-time PCR and immunohistochemistry,and podocyte density was calculated. [Results] Compared with normal group,there were differences between the model group(P<0.05). Two treatment groups compared with model group,BUN and 24h UTP significantly reduced(P<0.05),ITGA3, ITGB1,WT1 mRNA expression and podocyte density increased significantly (P<0.05). The pathological damage was significantly reduced in the intervention group,mainly manifested by the obvious decrease of cellular matrix and mesangial cells in the glomerulus. Compared with irbesartan group,Chinese medicine group of 24h UTP decreased obviously (P<0.05),ITGB1 mRNA expression and podocyte density increased significantly (P<0.05). [Conclusion] Chinese Herbs for stasis removing and collaterals dredging has a protective effect on the kidney of diabetic nephropathy rats and its mechanism may be associated with regulation of integrin α3β1 expression and thereby reducing podocyte shedding. |
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