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| Study on intestinal absorption characteristics of 4 components of total glucosides in picrorhiza scrophulariiflora by in situ singl-pass intestinal perfusion method |
| Hits 422 Download times 486 Received:November 12, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2022.12.17 |
| Key Words
in situ single-pass intestinal perfusion method;total glucosides in picrorhiza scrophulariiflora;intestinal absorption characteristics |
| Author Name | Affiliation | E-mail | | ZHANG Wanjing | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CAO Ningning | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Xiaoxuan | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | PEI Shuai | Discovery Chemistry Unit, HitGen Inc., Chengdu 610200, China | | | CAI Nan | Traditional Chinese medicine Research Center, Tasly Holding Group Co., Ltd., Tianjin 300410, China | alfalfacn@126.com | | XIAO Xuefeng | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | kai1219@163.com |
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| Abstract
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| [Objective] To investigate the absorption characteristics of picrosideⅠ,picrosideⅡ,picroside Ⅳ and androsin in different intestinal segments of rats,and explore the intestinal absorption characteristics of four components of total glucosides in Picrorhiza scrophulariiflora (TGPS). [Methods] The in situ single pass intestinal perfusion method was used,and an HPLC system was utilized to determine the contents of four components of TGPS. The absorption characteristics of four components of TGPS in duodenum,jejunum, ileum and colon were investigated with the absorption rate constant (Ka) and the effective permeability coefficient (Peff) as evaluation indexes. [Results] The Ka and Peff of four components from TGPS in different intestinal segments of rats were respectively all greater than 1.65×10-2/min,2.0×10-5cm/min,indicating that the absorption rate and absorption capacity of the four components in different intestinal segments were good,but there were some differences in the absorption rate and absorption capacity of each component in different intestinal segments. There was no notable difference in in Ka and Peff of picroside Ⅰ in the different intestinal segments (P>0.05), suggesting that there was no main absorption site of picroside Ⅰ;the best absorption sites for picroside Ⅱ,picroside Ⅳ and androsin were the duodenum,jejunum and colon,respectively,and the differences between different intestinal segments were statistically significant (P<0.05). In addition,compared with the other three components,there were significant differences in Ka and Peff of androsin in different intestinal segments (P<0.05),and the absorption of androsin in different intestinal segments was the best. [Conclusion] The study investigated the absorption characteristics of four components of TGPS in different intestinal segment,so as to provide reference for the clinical rational use of TGPS. |
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