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| Mechanism of abdominal massage regulating Tryptase-PAR2-PKCε pathway to prevent and treat visceral pain in IBS-D |
| Hits 629 Download times 1327 Received:October 12, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.01.15 |
| Key Words
abdominal massage;diarrhea-predominant irritable bowel syndrome;visceral hypersensitivity;Tryptase;PAR2;PKCε |
| Author Name | Affiliation | E-mail | | LI Huanan | National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
Level3 Laboratory of Biological Effects of Tuina Manipulation, State Administration of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yuyan | National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHANG Xiaofan | National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHANG Wei | National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
Level3 Laboratory of Biological Effects of Tuina Manipulation, State Administration of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Fei | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | DONG Hua | National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
Level3 Laboratory of Biological Effects of Tuina Manipulation, State Administration of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Jingui | National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | wjg65tj@163.com |
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| Abstract
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| [Objective] To verify the effect of abdominal massage on the prevention and treatment of visceral hypersensitivity in rats with diarrhea-predominant diarrhea (IBS-D),and to clarify its action pathway and mechanism.[Methods] Forty SD male rats were randomly divided into blank group,model group,abdominal massage group and Western medicine group,10 rats in each group. The IBS-D rat model was established by the mother-infant separation method + chronic stress method. The abdominal wall withdrawal score was used to evaluate the intervention effect. Toluidine blue staining was used to observe the number and status of mast cells in colon tissue. The distribution of Tryptase particles and the expression of PAR2 and PKCε protein in colon tissue were detected by immunohistochemistry and western-blot,respectively.[Results] Compared with the model group,the AWR scores of the abdominal massage group and the western medicine group were significantly lower (P<0.05). Microscopically,the shape of MC in the model group was irregular,and the count and density were significantly higher than those in the blank group;compared with the model group,the count and density of mast cells decreased in the abdominal massage group and western medicine group (P<0.01). Immunohistochemistry:compared with the model group,the distribution of Tryptase granules in mast cells in the abdominal massage group and the Western medicine group was significantly decreased (P<0.01). Western-blot:compared with the model group,the abdominal massage group and the Western medicine group had significantly lower distribution of Tryptase particles (P<0.01). The expressions of PAR2 and PKCε were significantly decreased (P<0.01).[Conclusion] Abdominal massage acts on the abdomen,possibly by activating intestinal MC,regulating its degranulation,reducing the release of Tryptase,and through the Tryptase-PAR2-PKCε pathway,thereby improving the mechanism of intestinal hypersensitivity in IBS-D model rats. |
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