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| Study on the mechanism of protecting the myocardial injury in rats with hyperlipidemia myocardial ischemia-reperfusion injury based on lipid metabolomics |
| Hits 772 Download times 775 Received:October 25, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.01.16 |
| Key Words
QishenYiqi Dripping Pill;cardiac reperfusion injury;myocardial preservation;lipidomics |
| Author Name | Affiliation | E-mail | | ZHAO Congna | Tianjin Beichen Traditional Chinese Medicine Hospital, Tianjin 300400, China | | | LUO Yang | Second Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300250, China | | | FENG Limin | Second Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300250, China | flimintcm@163.com |
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| Abstract
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| [Objective] To investigated the effect of Qishen Yiqi Dropping Pills (QSYQ) on lipid metabolism in rats with hyperlipidemia myocardial ischemia-reperfusion injury based on the lipidomics method,to explore the protecting mechanism against myocardial ischemia-reperfusion injury.[Methods] Rat model of hyperlipidemia was induced by using high-fat diet,and the left anterior descending branch was ligated to induce the model of myocardial ischemia and reperfusion injury. Sham operation group,model group,QSYQ low,medium and high dose group(62.5,135,270 mg/kg) were set in the present study. Creatine kinase(CK),lactic acid dehydrogenase (LDH) and lipid level were checked 3 hours after I/R. TTC staining was used to examine the myocardial infarction(MI) area. The lipidomics study of myocardial tissue homogenates was performed by using the UPLC-Orbitrap mass spectrometry system.[Results] Compared with the sham group,the serum LDL,TC,CK and LDH in the model group were significantly increased (P<0.05),and the myocardial infarction was more serious;Compared with the model group,the serum CK content and MI area were significantly decreased in the QSYQM rats (P<0.05),and the LDL and TC were decreased significantly. The 32 cardiac lipid subclasses and 1 141 lipid molecules were identified by lipidomics. The results of different lipid molecules showed that there were 44 significantly different lipid molecules between the model group and the sham group. There were 13 different lipid molecules in the QSYQM and the model group,and 6 lipid molecules[Cer (d18:1/18:0)+H,PE (16:0p/22:4)+H,PE (18:2p/18:2)+H,LPC (18:0)+H,LPC (18:0)+Na,and PC (16:0/18:1)+HCOO] with a significant opposite trend with the model group after the treatment (P<0.05,VIP>1.0).[Conclusion] We found that QSYQ protects from myocardial ischemia and reperfusion injury in hyperlipidemia by improving cardiac lipid metabolism,and this research would further enriched the modern research on myocardial protection by QSYQ. |
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