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| HPLC characteristic fingerprint analysis and research of benchmark samples of famous classical prescription Zexie Decoction |
| Hits 816 Download times 802 Received:October 28, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.01.21 |
| Key Words
famous classical prescription;Zexie Decoction;benchmark sample;characteristic fingerprint;HPLC |
| Author Name | Affiliation | E-mail | | JIANG Hengli | Tianjin Key Laboratory of Chinese Medicine Decoction Piece Processing Technology Enterprise, China Medico Corporation, Tianjin 300301, China | | | ZHONG Xin | Tianjin Tsumura Pharmaceuticals Co., Ltd., Tianjin 300301, China | | | WANG Shuangshuang | Tianjin Key Laboratory of Chinese Medicine Decoction Piece Processing Technology Enterprise, China Medico Corporation, Tianjin 300301, China | | | MA Xianghui | Tianjin Key Laboratory of Chinese Medicine Decoction Piece Processing Technology Enterprise, China Medico Corporation, Tianjin 300301, China | | | LIU Ye | Tianjin Key Laboratory of Chinese Medicine Decoction Piece Processing Technology Enterprise, China Medico Corporation, Tianjin 300301, China | | | KANG Yajuan | Tianjin Key Laboratory of Chinese Medicine Decoction Piece Processing Technology Enterprise, China Medico Corporation, Tianjin 300301, China | | | HUANG Fu | Tianjin Tsumura Pharmaceuticals Co., Ltd., Tianjin 300301, China | | | ZHANG Yonghe | Tianjin Tsumura Pharmaceuticals Co., Ltd., Tianjin 300301, China | | | CAO Lijuan | Tianjin Key Laboratory of Chinese Medicine Decoction Piece Processing Technology Enterprise, China Medico Corporation, Tianjin 300301, China | caolijuan@linmura.com.cn |
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| Abstract
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| [Objective] To establish the HPLC characteristic fingerprint of Zexie Decoction,analyze and evaluate its quality,so as to provide scientific reference and basis for the quality control standard.[Methods] According to the traditional method,the standard decoction of Zexie Decoction was prepared by the way of modern decoction method. The HPLC characteristic fingerprint of Zexie Decoction was established on Agilent ZORBAX SB-Aq C18 column(250 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile and water. The similarity and characteristic peak analysis of 18 batches Zexie Decoction were carried out by the “similarity evaluation system for chromatographic fingerprint of Chinese materia medica 2012 version”.[Results] The similarity of 18 batches of Zexie Decoction benchmark samples was 0.912~0.997,and 11 common peaks were marked. Four peaks were confirmed,they were alisol A(peak 4),atractylode Ⅰ(peak 6),alisol B(peak 8),23-acetyl alisol B(peak 9). The relative retention time of 11 characteristic peaks was specified with 23-acetyl alisol B (peak 10) as the S peak.[Conclusion] The established HPLC characteristic fingerprint analysis method was ingenious,stabilized,accurate and faithful,which could reflect the overall chemical composition characteristics of Zexie Decoction,and be used to provide basis for the quality evaluation of Zexie Decoction preparations. |
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