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| Optimization of extraction process of effective ingredients in Veratrum Nigrum L. |
| Hits 1170 Download times 1071 Received:November 11, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.02.17 |
| Key Words
Veratrum Nigrum L.;jervine;veratramine;JMP;process optimization |
| Author Name | Affiliation | E-mail | | ZHANG Mengmeng | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WEI Shujie | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SHEN Jiayuan | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | OUYANG Huizi | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yanxu | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HE Jun | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | hejun673@163.com |
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| Abstract
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| [Objective] With the help of the experimental design model and orthogonal experiment of JMP software,the extraction process of the active components of Veratrum Nigrum L. was optimized.[Methods] Single factor experiment and orthogonal experiment were used to optimize the ethanol volume fraction,material-to-liquid ratio,extraction time,and extraction times. The extraction process was determined by analyzing the content difference of jervine and veratramine.[Results] Optimal process obtained by the model is equal to the actual experimental results. The best process parameters as follows:the volume fraction of ethanol was 70%,2 hours each time,the extraction times were 3 times and ratio of liquid to material was 15:1 (mL/g). Under the optimized conditions,the contents of jervine and veratramine were 1.60 mg/g and 2.98 mg/g,and the RSD was 1.0% and 1.1%,respectively.[Conclusion] The extraction process was stable and could be used for the extraction of effective ingredients in Veratrum Nigrum L. |
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