|
| Curcumin-dopamine nanomedicines for combined chemo-photothermal therapy of glioma cell |
| Hits 1348 Download times 1531 Received:October 16, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2023.02.18 |
| Key Words
curcumin;glioma;nanomedicine controlled release;combined chemo-photothermal therapy |
| Author Name | Affiliation | E-mail | | JIANG Zhengping | Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China | | | ZHANG Yan | Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China | | | WANG Qiangsong | Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China | wangqs@bme.cams.cn |
|
| Abstract
|
| [Objective] To explore a drug delivery system to improve the poor water solubility of curcumin and rapid metabolism in vivo,and to achieve the purpose of curcumin-photothermal combination therapy for glioma cells.[Methods] Using the characteristics that dopamine hydrochloride can self-polymerize into polydopamine under weakly alkaline conditions,polydopamine nanoparticles (PDA@CUR NPs) entrapped curcumin were prepared to investigate the particle size,particle size stability,surface zeta potential,drug loading,entrapment efficiency,drug release and photothermal conversion properties of nanoparticles;at the cellular level,laser scanning confocal microscopy was used to investigate the in vitro simulation of PDA@CUR NPs crossing the blood-brain barrier and the uptake behavior of PDA@CUR NPs by glioma cells. Through cell proliferation and toxicity experiments,the combined therapeutic effect of PDA@CUR NPs on glioma cells was assessed.[Results] PDA@CUR NPs had a particle size of about 275 nm,uniform distribution and good stability,with a drug loading of (11.41±0.27)% and an entrapment efficiency of (81.73±2.22)% and the temperature could reach about 45℃ within 5 min under exogenous stimulation with near-infrared light. The photothermal and pH response of curcumin-loaded NPs was controlled and released. The results of cell experiments showed that PDA@CUR NPs could bypass the pored chamber(pore size 0.4 μm),glioma cells could uptake nanoparticles and the combination of curcumin and photothermal had a significant inhibitory killing effect on glioma cells.[Conclusion] PDA@CUR NPs can significantly improve the water solubility,poor stability and rapid metabolism of curcumin in vivo,providing a new idea for the combined treatment of glioma with chemotherapy combined with photothermal or other therapies. |
|
|
|
|
|
|
|
