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| Study on the protective mechanism of blumea balsamifera on acute liver injury based on animal experiment and network pharmacology |
| Hits 1126 Download times 1418 Received:November 20, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.02.19 |
| Key Words
Blumea balsamina;acute liver injury;network pharmacology;CCl4;mechanism |
| Author Name | Affiliation | E-mail | | GUAN Ningning | Key Laboratory of Ethnic Medicine, Ministry of Education, Minzu University of China, Beijing 100081, China | | | HUANG Yongze | Key Laboratory of Ethnic Medicine, Ministry of Education, Minzu University of China, Beijing 100081, China | | | CHEN Yue | Key Laboratory of Ethnic Medicine, Ministry of Education, Minzu University of China, Beijing 100081, China | | | BAI Hongyu | Key Laboratory of Ethnic Medicine, Ministry of Education, Minzu University of China, Beijing 100081, China | | | SONG Binbin | Key Laboratory of Ethnic Medicine, Ministry of Education, Minzu University of China, Beijing 100081, China | songbinbin0824@163.com | | HUANG Huoqiang | Key Laboratory of Ethnic Medicine, Ministry of Education, Minzu University of China, Beijing 100081, China | huanghuoqiang888@163.com |
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| Abstract
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| [Objective] This study was based on animal experiments and network pharmacology tostudy the protective effect and potential mechanism of Blumea balsamifera on acute liver injury(ALI).[Methods] Acute liver injury model was induced by intraperitoneal injection of CCl4 in mice. The protective effect of Blumea balsamifera on acute liver injury was preliminarily explored by observing the changes of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in mice. The network pharmacology method was used to retrieve the active components of Blumea balsamina,predict the action target,construct the "Drug-active component-target-disease" network and protein-protein Interaction Network(PPI),verify the docking between the obtained compounds and the targets by molecular docking method,and further explore the action mechanism of Blumea balsamina in the treatment of ALI by Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrich-ment analysis.[Results] The results showed that Blumea balsamina could significantly alleviate the acute liver injury induced by CCl4 and significantly reduce the levels of ALT and AST in mice(P＜0.05);The network pharmacology analysis showed that there were 36 main active componentsand 10 key components,including3-hydroxygenkwanin,luteolin,kaempferol,5,4'dihydroxy-7-methoxyflavone,Kumatakenin,Quercetin3,3'-dimethyl etheh,Chrysoeriol,Diosmetin,Eriodictyol, Pilloin. There were 171 targets for the action of Blumea balsamina,and 10 core targets were screened,including RAC-alpha serine/threonine-protein kinase (AKT1),vascular endothelial growth factor A (VEGFA),Epidermal growth factor receptor(EGFR),Estrogen receptor(ESR1),Caspase-3(CASP3),Interleukin-1 beta(IL1B),heat shock protein 90α family class A member1(HSP90AA1),Hypoxia-inducible factor 1-alpha(HIF1A),Mitogen-activated protein kinase 3(MAPK3),Protooncogene tyrosine-protein kinase Src(SRC). The results of moleculardocking showed that the key components and core targets screened bynetwork pharmacology could bind spontaneously. Go analysis shows that biological processes are mainly concentrated in protein phosphorylation,cellular response to nitrogen compound,positive regulation of cell migration,regulation of kinase activity. KEGG enrichment involves cancer pathway,PI3K-Akt signaling pathway,microRNA in cancer,lipid and atherosclerosis,MAPK signaling pathway and so on.[Conclusion]This study preliminarily confirmed that the extract of Blumeabalsamina has a significant protective effect on CCl4 induced acute liver injury,which may be through regulating AKT1,VEGFA and other core targets and cancer,PI3K-Akt and other signal path-ways,reducing inflammatory response and inhibiting oxidative stress,so as to produce liver protective effect. |
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