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| Effect of renzhong (GV26) electroacupuncture on endogenous neural stem cell differentiation in rats with cerebral infarction |
| Hits 519 Download times 3242 Received:November 08, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.03.10 |
| Key Words
cerebral infarction|electroacupuncture|renzhong (GV26)|endogenous neural stem cell|neurofunctional networks |
| Author Name | Affiliation | E-mail | | YIN Xiumei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | YANG Lihong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | HAN Jiawei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | CHEN Linling | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | QUI Tian | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | JIANG Tao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | DU Yuanhao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | jpjs_cn@sina.com |
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| Abstract
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| [Objective] The effect of renzhong(GV26) electroacupuncture on the proliferation and differentiation of endogenous neural stem cells after cerebral infarction(CI) was observed.[Methods] Wistar rats were randomly divided into electroacupuncture group,model group,sham surgery group and blank group,the first three groups were 24 in each group,divided into 4 subgroups according to 1,3,7 and 14 d,and 6 rats in blank group,and the degree of neurological deficit in each group was assessed by NSS. HE staining was used to observe the morphological changes of endogenous neural stem cells after CI. Immunohistochemistry and immunofluorescence double labeling to detect the proliferation and differentiation of endogenous neural stem cells after CI.[Results] In terms of neurological function,there was no change in the NSS score of the blank group and the sham surgery group;the neurological function was normal,and the CI rats in the model group and the electroacupuncture group improved their sensation,reflex and limb function with the extension of time,and the NSS scores of the electroacupuncture group at 3,7 and 14 d were lower than those in the model group,and the difference between the two groups was statistically significant (P<0.05);HE staining,blank group and sham surgery group showed no obvious abnormalities in brain tissue morphology,the model group and electroacupuncture group showed nerve cell nucleus contraction,with cell vacuoles,with the increase of intervention days,gradually connective tissue,astrocytes and normal cell production,the overall repair of electroacupuncture group was better than that of the model group. Compared with the blank group and sham group,Nestin AOD and GFAP AOD were significantly increased (P<0.01) in each time phase of rats in the model group,and Nestin AOD was higher (P<0.05 or P<0.01) in the electroacupuncture group compared with the model group,and GFAP AOD was increased (P<0.05 or P<0.01) at 3,7 and 14 d. Compared with the blank group and sham group,the expression of Nestin and GFAP in rats in the model group was significantly increased (P<0.01) and the fluorescence signal was strong,compared with the model group;the expression of Nestin in each phase of the electroacupuncture group was increased (P<0.05 or P<0.01);the Nestin/BrdU co-labeling signal was strong,the GFAP expression was elevated (P<0.05 or P<0.01) at 3,7 and 14 d,and the GFAP/BrdU co-labeling signal was strong.[Conclusion] Renzhong electroacupuncture can significantly improve the neurological damage of CI rats,promote the proliferation and differentiation of endogenous neural stem cells into astrocytes,and thus facilitate the repair and functional reconstruction of neural functional networks after CI. |
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