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| Metabolomics-based study of Shensu Ⅱ intervention in mouse with adriamycin-induced nephropathy |
| Hits 557 Download times 387 Received:December 20, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.03.14 |
| Key Words
Shensu Ⅱ|metabolomics|kidney injury|biomarker |
| Author Name | Affiliation | E-mail | | DOU Yitian | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinica Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | WANG Yuming | School of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangyuming226@163.com | | QU Jingtian | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinica Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | LI Xue | School of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Chong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinica Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | HAN Yanyan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinica Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | SHANG Yichun | School of Traditional Chinese Medicine, Tianjin University of Chinese Medicine, Tianjin 301617, China | | | LIU Chunliu | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| [Objective] To study the protective effect of ShensuⅡintervention on adriamycin-induced nephropathy in mice and its effect on plasma metabolism,and to preliminarily explore the metabolic pathways and possible mechanisms of its intervention.[Methods] A mouse nephropathy model was established by tail vein injection of adriamycin,and the morphological changes of kidney pathology were observed in each group after continuous administration for 14 d. The UPLC-Q-TOF/MS technique was applied to metabolomic study of plasma to screen and identify biomarkers associated with kidney injury and enrich the metabolic pathways to explore the possible mechanism of action of ShensuⅡ.[Results] ShensuⅡsignificantly improved the histopathological changes in the kidney of mice with nephropathy caused by adriamycin injection. The 14 potential biomarkers were screened by metabolomic analysis,involving amino acid metabolism,bile acid metabolism,lipid metabolism,fatty acid metabolism and purine metabolism and other metabolic pathways.[Conclusion] ShensuⅡcan effectively ameliorate the damage in adriamycin nephropathy mice,probably by affecting amino acid metabolism,bile acid metabolism and purine metabolism pathways. |
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