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| Study on UPLC fingerprint and content determination of 5 nucleosides in Shuxuetong Injection |
| Hits 614 Download times 390 Received:December 10, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.03.15 |
| Key Words
Shuxuetong Injection|UPLC|fingerprint|content determination |
| Author Name | Affiliation | E-mail | | CHAI Shiwei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medcine Acupuncture and Moxibastion, Tianjin 301617, China | | | GE Minglei | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WU Mengxuan | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HE Jun | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | hejun673@163.com |
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| Abstract
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| [Objective] To establish a UPLC fingerprint and a determination method of uracil,hypoxanthine,xanthine,inosine and guanine nucleoside in Shuxuetong Injection.[Methods] ACQUITY UPLC®BEH Amide column (2.1 mm×100 mm,1.7 μm) was used for gradient elution with 0.1% formic acid in water and acetonitrile as mobile phase. The column temperature was 25℃,the flow rate was 0.3 mL/min,and the detection wavelength was 254 nm. UPLC fingerprints of 33 batches of Shuxuetong Injection were established. Similarity analysis was conducted by using similarity evaluation software of Chinese medicine chromatographic fingerprint,and the contents of 5 nucleosides in the samples were determined.[Results] The methodology of the fingerprint and content determination methods of 5 nucleosides met the requirements. The similarity of different batches of Shuxuetong Injection was above 0.998. There were 10 common peaks in the fingerprints of 33 batches of samples,among which five components were identified,including uracil,hypoxanthine,xanthine,inosine and guanine nucleoside. The average content of uracil,hypoxanthine,xanthine,inosine and guanine was 34.57,153.50,52.76,53.23 and 14.46 μg/mL,respectively.[Conclusion] The established UPLC fingerprint and content determination method for Shuxuetong Injection is reliable and simple to operate,which can provide a certain reference for quality evaluation of Shuxuetong injection. |
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