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| Study on acupuncture regulating miR-10a to improve cognition and reduce neuroinflammation in VD rats |
| Hits 498 Download times 855 Received:January 25, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.04.11 |
| Key Words
acupuncture;vascular dementia;cognitive function;miR-10a;neuroinflammation |
| Author Name | Affiliation | | ZHANG Jie | Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Traditional Chinese Medicine Acupuncture, Tianjin 300381, China | | LIU Xiaoxi | Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Traditional Chinese Medicine Acupuncture, Tianjin 300381, China | | ZHANG Xuezhu | Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Traditional Chinese Medicine Acupuncture, Tianjin 300381, China |
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| Abstract
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| [Objective] To explore the effect and mechanism of acupuncture on improving cognitive function and reducing neuroinflammation in vascular dementia(VD) rats by regulating miR-10a. [Methods] The VD rat model was established by clamping bilateral common carotid arteries and divided into sham operation, VD, acupuncture and miR intervention groups. The acupuncture group was treated with "sanjiao acupuncture". Each miR intervention group was given corresponding miR-10a inhibitor NC or inhibitor. Morris water maze was used to evaluate the cognitive ability of rats;the expression of miR-10a was detected by RT-PCR;NF-κB total p65 and phosphorylated p65 expression were detected by Western blot;TNF-α and IL-6 levels were determined by ELISA. [Results] Compared with the sham operation group, the escape latency of the model group in the water maze hidden platform test was significantly prolonged (P<0.01). In the space exploration test, the number of times to cross the original platform position decreased, and the time required to cross the original platform for the first time increased(P<0.01). The acupuncture group had an improvement effect on the above indicators, which was significantly different from the model group(P<0.01). In addition, compared with sham operation group, the expression of miR-10a in hippocampus of model group decreased, and NF-κB t-p65 and p-p65 proteins and TNF-α, IL-6 level increased (P<0.05 or P<0.01).After acupuncture treatment, the above indexes showed opposite changes, which were significantly different from the model group(P<0.05 or P<0.01). After transfection with miR-10a inhibitor, increased levels of NF-κB t-p65 and p-p65 proteins and TNF-α, IL-6 was found in the hippocampus of the inhibitor group when compared with the model group(P<0.05 or P<0.01).Aacupuncture treatment could improve the cognitive function of rats in miR-10a inhibitor NC group and reduce the NF-κB protein and TNF-α, IL-6 level, but no such effect was found in the inhibitor group. Double luciferase reporter gene experiment found that miR-10a can directly act on NF-κB and inhibit its expression. [Conclusion] Acupuncture can improve the cognitive function of VD rats. Its mechanism is partly related to regulating the expression of miR-10a, and then inhibiting the inflammatory damage of ischemic brain tissue. |
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