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| Effects of total saponins of pangolin on lung function, lung tissue inflammation and airway remodeling in asthmatic rats through JAK2/STAT3 pathway |
| Hits 1191 Download times 1251 Received:December 15, 2022 |
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| DOI
10.11656/j.issn.1672-1519.2023.04.19 |
| Key Words
asthma;epithelial cell damage;total saponin from rhizoma dioscorea nipponica;JAK2/STAT3 signaling pathway |
| Author Name | Affiliation | E-mail | | WANG Zhi | Racecourse Ward Respiratory Department, Tianjin Children's Hospital, Tianjin 300202, China | | | WU Lei | Department of Respiratory Medicine, Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang 050013, China | shi357325@163.com |
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| Abstract
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| [Objective] To investigate the effects of total saponin from rhizoma dioscorea nipponica on airway epithelial cell injury in asthmatic rats and its regulatory effects on Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling pathway. [Methods] The asthmatic rat model was established by ovalbumin induction. The rats were randomly divided into model group, total saponin from rhizoma dioscorea nipponica low-dose group, total saponin from rhizoma dioscorea nipponica medium-dose group and total saponin from rhizoma dioscorea nipponica high-dose group, and the normal control group was set up. The levels of interleukin (IL)-4, IL-6 and interferon-γ(IFN-γ) in bronchial lavage fluid were determined by ELISA. The pathological damage of bronchial tissue was observed by HE staining. The mRNA levels of transforming growth factor-β1(TGF-β1) and Smad2 in bronchial tissue were detected by qRT-PCR. The relative expressions of p-JAK2 and p-STAT3 were detected by Western blotting. [Results] The lung tissue structure of rats in normal control group was intact without inflammatory cell infiltration. In the model group, inflammatory neutrophil infiltration, interstitial edema, alveolar septal thickening, patchy hemorrhage in alveolar and interstitial tissues were observed. The pathological injury of lung tissue in low, medium and high dose total saponin from rhizoma tioscorea nipponica groups was alleviated;compared with the normal control group, the lung compliance of the model group, the lung elastic resistance, the levels of IL-4, IL-6 and IFN-γ, the mRNA levels of TGF-β1 and Smad2 were increased, and the relative protein expressions of p-JAK2/JAK2 and p-STAT3/STAT3 were increased (P<0.05). Compared with model group, lung compliance was increased, lung elastic resistance, IL-4, IL-6 and IFN-γ levels, TGF-β1 and Smad2 mRNA levels, and p-JAK2/JAK2 and p-STAT3/STAT3 protein relative expression levels were decreased in total saponin from rhizoma dioscorea nipponica low-dose, medium-dose and high-dose groups (P<0.05). The most significant changes were observed intotal saponin from rhizoma dioscorea nipponica high-dose group, followed by total saponin from rhizoma dioscorea nipponica medium-dose group and total saponin from rhizoma dioscorea nipponica low-dose group (P<0.05). [Conclusion] Total saponin from rhizoma dioscorea nipponica can reduce airway epithelial cell injury, reduce inflammation, improve airway remodeling, enhance lung compliance, and reduce lung elastic resistance in asthmatic rats, which may play a role by inhibiting JAK2/STAT3 signaling pathway. |
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