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| Effect of Wuji Baifeng Tablets on premature ovarian insufficiency in mice induced by VCD |
| Hits 581 Download times 339 Received:March 16, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.06.15 |
| Key Words
Wuji Baifeng Tablets;VCD;primary ovarian insufficiency;FSH;LH |
| Author Name | Affiliation | E-mail | | LU Xingyue | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Na | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GAO Panwei | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YUAN Qing | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MIAO Lin | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Han | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yi | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHAI Lijuan | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | cljuan1258@163.com |
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| Abstract
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| [Objective] Establish the mouse model of primary ovarian insufficiency（POI） with 4-deoxyvinylcyclohexene（VCD） and explore the therapeutic effect of Wuji Baifeng Tablets on VCD-induced POI mice. [Methods] 1）The POI mouse model was established：sixty 8 week old Kunming female mice were randomly divided into 30 control （CON） mice and 30 model （MOD） mice. In MOD group，the POI model was reproduced by intraperitoneal injection of 160 mg/（kg·d） VCD，and CON was injected with sesame oil of the same volume. During the modeling period，the estrus cycle was observed by vaginal smear every day；from day 15 to day 65 of modeling，blood was taken from orbit every 5 days，and serum follicle stimulating hormone （FSH） and luteinizing hormone （LH） levels were detected by ELISA. At 45 to 65 days after modeling，the uterus and ovary of mice were taken every 5 days，and the organ index was calculated after weighing，and morphological changes in the ovaries were observed by hematoxylin-eosin （HE） staining. 2）Therapeutic effect of Wuji Baifeng Tablets on POI mice：Another sixty 8 week old female Kuming mice were selected according to the above modelling method. After successful modeling，they were randomly divided into MOD group，estradiol valerate （E2） group [0.3 mg/（kg·d）]，and low，medium and high dose Wuji Baifeng Tablets groups[410，820，1640 mg/（kg·d）]，with 10 mice in each group. Once daily for 45 consecutive days，the estrus cycle of mice was observed during administration. The mice were killed on day 46 of administration，and serum FSH and LH levels were detected by ELISA. The uterus and ovaries of mice were taken，and the viscera index was calculated after weighing. The morphological changes in ovaries were observed by HE staining. [Results] 1）Establishment of mouse POI model：compared to the CON group，estrous cycle disorder in the MOD group lasted for 4 weeks on day 55. At day 50 and day 55 of VCD modeling，serum FSH and LH in MOD mice increased significantly twice. The wet weight and organ index of uterus and ovary decreased significantly，and the number of growing follicles decreased significantly. 2）Therapeutic effect of Wuji Baifeng Tablets on POI mice：45 days after intragastric administration，compared with the CON group，the estrous cycle of mice in the MOD group was disordered，and serum FSH and LH were significantly increased. Compared with the MOD group，the estrus cycle of mid-dose and high-dose Wuji Baifeng Tablets returned to normal，and serum FSH and LH of mice in each Wuji Baifeng Tablets dose group decreased significantly. The wet weight and organ index of uterus and ovary decreased significantly，and the number of primordial follicles in the ovary increased significantly. [Conclusion] After 15 days of intraperitoneal injection of 160 mg/（kg·d） VCD，a mouse model of POI could be prepared after 40 days of observation，and a total of 55 days of modeling. Wuji Baifeng Tablets may reduce serum FSH and LH levels by regulating the hypothalamus pituitary ovary axis （HPOA） in mice，regulating the estrous cycle of mice，and promoting follicular development to treat mouse POI caused by VCD. |
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