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| Study on the anti breast cancer effect and mechanism of Xihuang Pill based on SIRT1/SOX2 signal pathway |
| Hits 780 Download times 352 Received:February 17, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.06.16 |
| Key Words
Xihuang Pill;silence regulatory protein 1/sex determining region Y box protein 2 signal pathway;breast cancer;proliferation;transfer |
| Author Name | Affiliation | E-mail | | WU Haibin | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | | | MA Zhiqiang | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | | | LI Shuai | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | | | ZHANG Guannan | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | | | MIAO Yayun | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | | | ZHAN Ximei | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | | | WANG Wensheng | Department of Breast and Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng 475100, China | 517508474@qq.com |
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| Abstract
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| [Objective] To explore the anti-breast cancer effect and mechanism of Xihuang pill based on silencing regulatory protein 1 (SIRT1)/sex determination region Y frame protein ２ （ＳＯＸ２） ｓｉgnaling pathway. [Methods] MDA-MB-231 cells were grouped into control group，low dose Xihuang pill grouｐ （５ g/L），medium dose Xihuang Pill group （7.5 g/L），high dose Xihuang Pill group （15 g/Ｌ），SRT 1 720（SIRT1 activator） group （6 μmol/Ｌ），high dose Xihuang pill+SRT 1720 group （15 g/L+6 μmol/L）；CCK-8 method and plate cloning test were used to detect cell proliferation；cell migration was detected by scratch test；transwell method was used to detect cell invasion； Western blot was used to detect the expression of proliferating cell nuclear antigｅｎ（ＰＣＮＡ），matrix metalloproteinａｓｅ （ＭＭＰ-2），ＭMP-9， SIRT1，and SOX2 in cells；the tumor volume，mass，quality and PCNA，MMP-2，MMP-9，SIRT1，SOX2 protein expression of nude mice in each group were measured in vivo tumor transplantation experiment. [Results] Xihuang Pill inhibited the proliferation，migration and invasion of MDA-MB-231 cells and the growth of transplanted tumor in nude mice in a dose-dependent manner；SIRT1 activator SRT 1 720 promoted the proliferation，migration and invasion of MDA-MB-231 cells and the growth of transplanted tumor in nude mice；the high dose Xihuang Pill plus SIRT1 activator group attenuated the inhibitory effect of high dose Xihuang Pill on the proliferation， migration，invasion of MDA-MB-231 cells and the growth of transplanted tumor in nude mice. At the same time，Xihuang Pill inhibited the expression of SIRT1 and SOX2 proteins in MDA-MB-231 cells and transplanted tumor in nude mice in a dose-dependent manner， inhibited the activity of SIRT1/SOX2 signal pathway，and inhibited the proliferation，migration，invasion of MDA-MB-231 cells and the growth of transplanted tumor in nude mice by reducing the expression of PCNA，MMP-2 and MMP-9. [Conclusion] Xihuang Pill may inhibit the proliferation，migration and invasion of MDA-MB-231 cells and the growth of transplanted tumor in nude mice by inhibiting SIRT1/SOX2 signal pathway. |
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