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| Effect of micheliolide on apoptosis of human astrocytoma cells |
| Hits 415 Download times 275 Received:March 20, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.06.17 |
| Key Words
glioma;apoptosis;oxidative stress;micheliolide |
| Author Name | Affiliation | E-mail | | HUANG Wenlong | The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China
People's Hospital of Ganzhou City, Ganzhou 341000, China | | | YUAN Ze | Clinical Laboratory of PKUCare Luzhong Hospital, Zibo 255499, China
Southwest Medical University, Luzhou 646099, China | | | PENG Weiwei | People's Hospital of Ganzhou City, Ganzhou 341000, China | | | LIU Guosheng | People's Hospital of Ganzhou City, Ganzhou 341000, China | | | XUE Yingxian | Department of Pathology, Zibo Central Hospital, Zibo 255036, China | | | LI Fei | People's Hospital of Ganzhou City, Ganzhou 341000, China | leephialf@126.com | | LI Ning | Clinical Laboratory of PKUCare Luzhong Hospital, Zibo 255499, China | 2801084687@qq.com | | LIU Jiansheng | People's Hospital of Ganzhou City, Ganzhou 341000, China | gzliujs@126.com |
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| Abstract
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| [Objective] To explore the effects of micheliolide （MCL） on the apoptosis of human astrocytoma cells （U87） and its mechanism. [Methods] CCK-8 assay was used to detect the inhibitory effect of MCL on the growth of U87 cells；Annexin V-FITC/PI double staining were performed to detect the apoptosis of U87 cells induced by MCL. Mitochondrial membrane potential of U87 cell effected by MCL was measured by mitochondrial membrane potential assay kit with JC-1；2’，7’-dichlorodihydrofluorescein diacetate （DCFH-DA） fluorescent probe was used to detect the level of reactive oxygen species （ROS） in U87 cells effected by MCL；the contents of malondialdehyde （MDA），superoxide dismutase （SOD），trace reduced glutathione （GSH） and nitric oxide （NO） content of MCL on U87 cells were detected by biochemical methods. RT-qPCR was used to detect Caspase-3，Bax，Bcl-2 mRNA expression in in U87 cells effected by MCL. The specific binding sites of MCL with Caspase-3，Bax and Bcl-2 were found by molecular docking. [Results] The growth of U87 cells was inhibited by MCL in a dose-dependent manner，and the IC50 values of MCL against U87 cells were around 11.27 μmol/L；and the apoptosis level showed that a significant increase in the apoptosis rate of U87 cells when treated with 20 μmol/L MCL（P<0.001）. The mitochondrial membrane potential of U87 cells was significantly reduced when administered in the 5，10 and 20 μmol/L group（P<0.001）. The results of oxidative stress index showed that MCL was dose-dependent and upregulated the ROS，MDA， NO content in U87 cells，and down-regulated SOD and GSH （P<0.05）. The expression levels of Caspase-3 and Bax mRNA in U87 cells were significantly upregulated（P<0.01） when administered in the 20 μmol/L group （P<0.01），while the expression levels of Bcl-2 mRNA were significantly downregulated （P<0.05）. The molecular docking results showed that MCL formed stable hydrogen bonding with Caspase-3，Bcl-2 and Bax amino acid residues ARG-164，LEU-168 and ARG-66. [Conclusion] MCL significantly inhibit U87 cell growth，mediate high oxidative stress levels，reduce mitochondrial membrane potential and promote apoptosis. |
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