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| Effect and mechanism of Qishen Yiqi Droplet on liver injury induced by high-fat diet combined with streptozotocin in diabetes mice |
| Hits 550 Download times 338 Received:May 03, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.07.15 |
| Key Words
Qishen Yiqi Droplet;diabetes;liver injury;hepatocyte apoptosis;steatosis |
| Author Name | Affiliation | E-mail | | LIU Xin | Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China | | | LIU Tiantian | Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China | liutiantian008@126.com | | ZHANG Yanli | Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China | | | GONG Ying | Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China | | | GU Yuanyuan | Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China | | | CAO Junling | Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China | caojunling72@163.com。 |
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| Abstract
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| [Objective] To explore the protective effect and mechanism of Qishen Yiqi Droplets on liver injury in diabetes mice. [Methods] Eighty male mice were randomly divided into control group，model group，Qishen Yiqi Droplets low-dose group（227.5 mg/kg），high-dose group（455 mg/kg），EX-527 inhibitor group. The control group was fed with normal diet，and the other four groups were fed with high-fat diet. From the third week，50 mg/kg streptozotocin was intraperitoneally injected for 7 consecutive days to establish the liver injury model of type 2 diabetes. The control group was injected with equal dose of normal saline. In the fifth week，14 days after Qishen Yiqi Droplets intervention，serum was taken to detect the levels of AST，ALT，TC and TG. The morphology and structure of the liver，the area of collagen fibers，the degree of hepatic steatosis and the number of hepatocyte apoptosis were detected by pathological staining. The expressions of Sirt1，AMPK and PGC-1α were measured by western blot. [Results] Compared with the control group，the serum AST，ALT，TC and TG of diabetes mice were significantly increased （P<0.01），the structure of hepatic lobule was disordered，the nucleus was deeply stained，pyknotic，local inflammatory infiltration，there were a lot of red fat droplets in hepatocyte，the area of liver fibrosis and the number of apoptotic hepatocyte were significantly increased（P<0.01）. The p-AMPKα/AMPKα expression reduced （P<0.01），the content of Sirt1 and PGC-1α was increased significantly （P<0.05 or P<0.01）. Both the low and high dose groups of Qishen Yiqi Droplets can diminish liver function damage indicators（P<0.05 or P<0.01），inhibit the pathological changes of liver structure and liver steatosis，alleviate liver fibrosis damage，reduce the number of hepatocyte apoptosis （P<0.01），and improve Sirt1 and p-AMPKα/AMPKα level （P<0.05 or P<0.01），abate PGC-1α expression（P<0.01）. EX-527 partially reversed the protective effect of Qishen Yiqi Droplets. [Conclusion] Qishen Yiqi Droplets may activate Sirt1/AMPKα/PGC-1α signal，inhibit hepatic fibrosis in diabetes mice，reduce liver steatosis and hepatocyte apoptosis，and then promote the repair of liver function in diabetes. |
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