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| Simultaneous determination of 10 components in Zhishi Xiebai Guizhi Decoction by UHPLC-MS/MS |
| Hits 525 Download times 371 Received:May 04, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.07.16 |
| Key Words
UHPLC-MS/MS;Zhishi Xiebai Guizhi Decotion;content determination;chemical composition |
| Author Name | Affiliation | E-mail | | ZHANG Xiaoying | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | QI Jige | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SU Shijia | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Yingli | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | | | ZHOU Kun | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | | | ZHANG Yue | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | zhyjoy1111@126.com。 |
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| Abstract
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| [Objective] To establish a method for the determination of quercetin，apigenin，protocatechuic acid，luteolin，syringin，ferulic acid，naringin，magnolol，hesperidin and neohesperidin in Zhishi Xiebai Guizhi Decotion by UPHLC-MS/MS to provide a reference for their quality control. [Methods] The constituents separation was carried on Welchultimate XB-C18 column （2.1 mm×100 mm，3 μm） with a mobile phrase of 0.1% formic acid in water and acetonitrile，gradient elution，the injection volume was 3 μL，the flow rate was 0.2 mL/min，and the column temperature was 40 ℃. ESI ion source，positive and negative ion switching mode，multiple reaction detection （MRM） mode were employed. [Results] The contents of 10 components in Zhishi Xiebai Guizhi Decotion were measured. The results of the methodological investigation of the quantitative analysis conditions were good（r>0.995），the RSD of the precision，stability and reproducibility tests were less than 2.86%，and the recovery of the spiked samples were between 97.68% and 101.66%. [Conclusion] The method is simple，rapid，accurate and reliable，and can be used for the quality control of Zhishi Xiebai Guizhi Decotion. |
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