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| Effects of Wendan Decoction on lipid metabolism in mice with metabolism-related fatty liver disease |
| Hits 450 Download times 518 Received:April 18, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.08.16 |
| Key Words
Wendan Decoction;metabolically associated fatty liver disease;lipid metabolism;lipid peroxidation;pioglitazone |
| Author Name | Affiliation | | LI Yupeng | Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, Tianjin 300120, China | | ZANG Chaoyue | The First Affiliated Hospital of Tianjin University of Tradition Chinese Medicine, Tianjin 300381, China |
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| Abstract
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| [Objective] To investigate the effect of Wendan Decoction on drug treatment in metabolically related fatty liver disease (MAFLD) mice and the potential molecular mechanism. [Methods] After 1 week of adaptive feeding,44 C57BL/6J male mice were randomly divided into control group,model group,Wendan Decoction low-dose group (low-dose group),Wendan Decoction medium-dose group (medium-dose group),Wendan Decoction high-dose group (high-dose group) and pioglitazone group. The mice were given high-fat diet for MAFLD modeling. The low,medium and high dose groups were treated at 1.3,2.6 and 5.2 g/kg every day,respectively,and pioglitazone group was treated at 2.6 mg/kg every day. All mice in treatment groups were given intragastric administration for 6 weeks,once a day. The mice were killed at the end of the 14th week. The liver index,pathological changes of liver tissue and fat content of the 6 groups of mice were compared. The levels of serum transaminase and liver fat,malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue and the expression levels of the important genes and proteins of lipid metabolism,including SREBP-1c,FAS,DGAT-2,SCD-1 and ACO were detected. [Results] Compared with blank group,the overall state of mice was poor,the liver tissue was large area steatosis and lipid infiltration,serum transaminase and liver fat levels were significantly increased in model group,which confirmed that the MAFLD model of mice were successfully constructed after 8 weeks of high fat diet. Compared with model group,the liver index,liver pathological changes,liver fat level,serum transaminase,lipid peroxidation level and the expression of important genes and proteins in lipid metabolism of mice in all treatment groups were significantly decreased,the most significant decrease were in high-dose and pioglitazone groups,the differentiation of each group results were statistically significant (P<0.05). [Conclusions] Different doses of Wendan Decoction can significantly improve the liver lipid deposition,liver function and lower the liver index of MAFLD mice,which may be related to the regulation of lipid metabolism,the reduction of lipid peroxidation level in liver tissue and the expression of important genes and proteins in lipid metabolism. |
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