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| Study on the effect and mechanism of Buyang Huanwu Decoction on improving aspirin induced gastrointestinal injury by intervention of intestinal microflora |
| Hits 378 Download times 383 Received:March 18, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.08.17 |
| Key Words
aspirin;digestive tract injury;intestinal flora;Buyang Huanwu Decoction;antiplatelet drugs |
| Author Name | Affiliation | | LU Meijuan | Cardiorascular medicine, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | YANG Peili | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | LIU Yi | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
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| Abstract
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| [Objective] To evaluate the antiplatelet effect of Buyang Huanwu Decoction and explore its intervention effect and mechanism of antiplatelet drug-induced gastrointestinal injury from the perspective of intestinal flora based on 16SrDNA technology. [Methods] The 75 clean grade healthy male SD rats were divided into control group,model group,Buyang Huanwu Decoction group,Xuefu Zhuyu Decoction group and Shuangkang group,with 15 rats in each group. The control group was fed normally,and the other four groups were given corresponding drugs by gavage on the basis of aspirin for 2 weeks. The levels of serum arachidonic acid(AA),thromboxane B2 (TXB2) and platelet membrane glycoprotein Ⅱb/Ⅲa(GPⅡb/Ⅲa) were measured. The body weight change,clotting time,fecal occult blood test and pathological sections of digestive tract mucosa were observed in each group. DNA from rat feces was extracted for 16SrRNA sequencing and analysis. [Results] Compared with the model group,the levels of AA and TXB2 in Buyang Huanwu Decoction group decreased,but the difference was not statistically significant(P>0.05). Compared with the control group,the level of GPⅡb/Ⅲa in the Buyang Huanwu Decoction group was significantly lower(P<0.01). The positive rate of fecal occult blood of rats in Buyang Huanwu Decoction group was the lowest(P<0.05). And the coagulation time in vitro,weight gain and pathological injury of intestinal mucosa were improved to varying degrees;In addition,according to the diversity analysis of Alpha and Beta,Buyang Huanwu Decoction can improve the abundance and diversity of digestive tract flora at the level of phylum and genus. [Conclusion] Buyang Huanwu Decoction has antiplatelet effect,can significantly reduce the level of GP Ⅱb/Ⅲa,and can improve the digestive tract injury caused by aspirin. Its protective effect on the digestive tract may be related to affecting the abundance and diversity of the digestive tract flora,improving the ratio of Thick-walled bacteria to Bacteroides,and up-regulating the genera of Platypiella and Lactobacillus. |
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