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| Discussion on the mechanism of Yiqi Yangyin Tongluo Recipe in anti-rheumatoid arthritis based on network pharmacology-molecular docking |
| Hits 347 Download times 355 Received:May 23, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.08.20 |
| Key Words
Yiqi Yangyin Tongluo Recipe;rheumatoid arthritis;network pharmacology;molecular docking;PI3K-AKT signaling pathway |
| Author Name | Affiliation | E-mail | | ZHANG Bolun | Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | | | JU Baozhao | Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | jubaozhao@163.com | | ZHANG Mi | Qingdao Sixth People's Hospital, Qingdao 266000, China | | | GAO Mingli | Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | | | JIANG Quan | Guang'anmen Hospital, Chinese Academy of Traditional Chinese Medicine, Beijing 100000, China | | | YU Jing | Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | | | SHEN Kong | The Second Outpatient Department of the General Hospital of the Northern Theater Command, Shenyang 110015 | 2632175778@qq.com | | ZHAO Yeyu | Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | | | QI Qing | Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | | | GUAN Rui | Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China | |
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| Abstract
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| [Objective] To explore the mechanism of Yiqi Yangyin Tongluo Recipe in intervention of rheumatoid arthritis(RA) by network pharmacology and in vitro experiments. [Methods] In the network pharmacology,first of all,we got the compound active ingredients and targets from TCMSP,HERB and literature,the RA targets from the Genecards and OMIM databases,and the cross-targets of the compound ingredients and RA. Thirdly,the function and pathway enrichment analysis of the intersection target were performed on the Metascape platform,and the molecular docking between the main active ingredients and the core target was performed. [Results] A total of 83 active ingredients,779 potential targets of compound prescriptions,5 393 RA-related targets and 465 intersection targets,including 9 intersection core targets (SRC,STAT3,MAPK3,MAPK1,HSP90AA1,PIK3R1,GRB2,AKT1,PIK3CA respectively) were screened by network pharmacology. Enrichment analysis showed the PI3K-AKT,cancer-related and neuroactive ligand-receptor interaction pathways,among which PI3K-AKT signaling pathway may be one of the key pathways. The results of molecular docking showed that the 11 main active components of Yiqi Yangyin Tongluo Recipe had relatively stable binding to the key targets SRC,STAT3,PIK3R1,and AKT1. Among them,the combination with AKT may be the largest one. [Conclusion] Yiqi Yangyin Tongluo Recipe intervenes RA through a multi component-target-tongluo approach. PI3K-AKT,cancer-related and neuroactive ligand-receptor may all be the key interaction pathways. |
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