|
| Effect of cedarol on proliferation，migration and invasion of breast cancer cells by regulating miR-503-5p/TCF3 axis |
| Hits 374 Download times 254 Received:April 18, 2023 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2023.09.16 |
| Key Words
cedarol;miR-503-5p/TCF3 axis;breast cancer;proliferation;migration;cell invasion |
| Author Name | Affiliation | | FENG Yanzhi | Department of Breast Surgery, People's Hospital of Zhengzhou, Zhengzhou 450099, China | | NIU Bing | Department of Breast Surgery, People's Hospital of Zhengzhou, Zhengzhou 450099, China | | YE Beibei | Department of Breast Surgery, People's Hospital of Zhengzhou, Zhengzhou 450099, China |
|
| Abstract
|
| [Objective] To investigate the effect of cedarol on the proliferation，migration and invasion of breast cancer（BC） cells by regulating miR-503-5p/T cell factor 3（TCF3） axis. [Methods] The expression levels of miR-503-5p and TCF3 protein in BC tissues and cells were detected by real-time fluorescent quantitative PCR（qRT-PCR） and Western blot respectively；MCF7 cells were treated with different concentrations of cedarol（0，14，28，56，112，225 μmol/L）. Cell proliferation was detected by MTT method；MCF7 cells were grouped into control group（normal culture），cedarol group（112 μmol/L），inhibitor-NC（transfected with inhibitor-NC），miR-503-5p inhibitor group（transfected with miR-503-5p inhibitor）. The expression level of miR-503-5p in cells was detected by qRT-PCR；cell proliferation，migration and invasion were detected by MTT method，Edu staining and Transwell cell，respectively；Western blot was applied to detect the expression of proliferating cell nuclear antigen（PCNA），matrix metalloproteinase-2（MMP-2） and TCF3 proteins；the target relationship between miR-503-5p and TCF3 was verified by double luciferase reporter gene experiment；the effect of cedarol on the growth of BC tumor and the miR-503-5p/TCF3 axis was verified by the tumor formation experiment in mice. [Results] In BC tissues and cells，the expression level of miR-503-5p decreased and the expression level of TCF3 protein increased（P＜0.05）. Cedarol inhibited the proliferation，migration and invasion of MCF7 cells and decreased the expression of PCNA，MMP-2 and TCF3 proteins in MCF7 cells（P＜0.05），inhibition of miR-503-5p expression could reverse the inhibitory effects of cedarol on the proliferation，migration and invasion of MCF7 cells（P＜0.05）；the double luciferase reporter gene experiment confirmed that miR-503-5p targeted the expression of MCF7（P＜0.05）；the results of tumor-bearing experiment in mice showed that cedarol could reduce the weight and volume of transplanted tumors，increase the level of miR-503-5p in transplanted tumor，and reduce the level of TCF3 and MMP-2 expression（P＜0.05）. [Conclusion] Cedarol can inhibit the proliferation，migration and invasion of BC cells，and its mechanism may be related to the regulation of miR-503-5p/TCF3 axis，which may become a new target for the treatment of BC. |
|
