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| Effect of the method of resolving phlegm and dispersing stasis on blood lipids and atherosclerosis in ApoE-/- mice |
| Hits 337 Download times 221 Received:May 28, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.10.15 |
| Key Words
atherosclerosis;hyperlipidemia;method of resolving phlegm and dispersing nodules;Neixiao Ruanmai Decoction No.2;low density lipoprotein cholesterol receptor;uncoupling protein 1 |
| Author Name | Affiliation | E-mail | | LI He | The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | | JI Hanrui | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | DU Yawei | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | WU Shengxian | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | wushx@sina.com |
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| Abstract
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| [Objective] To explore the effect of Neixiao Ruanmai Decoction No.2 of Huatan sanjie treatment，a prescription for resolving phlegm and dispersing stasis，on blood lipids and atherosclerosis in ApoE-/- mice，and explore its mechanism. [Methods] The 30 ApoE-/- mice were randomly divided into three groups on average. They were fed with high-fat diet for 16 weeks to establish atherosclerosis model. They were simultaneously given distilled water，low-dose[2.1 g/（kg·d）]and high-dose [4.2 g/（kg·d）] of Neixiao Ruanmai Decoction No.2 by gavage for 16 weeks. Four items of blood lipid were detected by biochemical method. The morphology of aortic plaque，scapular adipose tissue and liver were observed by HE staining，Western blot method was used to detect the expression of uncoupling protein1（UCP1） in scapular adipose tissue and low-density lipoprotein receptor（LDLR） in liver to evaluate the effect of Neixiao Ruanmai Decoction No.2 on improving atherosclerosis and lowering blood lipid，and to preliminarily explore the mechanism of action of the drug. [Results] Compared with the control group，the area of aortic plaque in the low-dose and high-dose groups of Neixiao Ruanmai Decoction No.2 decreased，while the serum LDL-C and TC in the low-dose group decreased（P＜0.05） and HDL-C increased（P＜0.05）；In the high-dose group，serum LDL-C and TC decreased（P＞0.05） and HDL-C increased（P＜0.05）. The results of oil red O staining showed that the liver steatosis in both low and high dose groups significantly improved. The results of HE staining showed that the scapular adipose tissue of low and high dose groups was activated. Western blot results showed that both low-dose and high-dose groups could increase the expression of UCP1 in liver LDLR and scapular adipose tissue（P＜0.05）. [Conclusion] Huatan Sanjie treatment can improve the atherosclerosis and hyperlipidemia of ApoE-/- mice，and up-regulate the expression of LDLR in liver and UCP1 in scapular adipose tissue may be its potential mechanism. |
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