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| The effect of membranous kidney No.1 formula on renal pathology and PI3K/Akt/mTOR signaling pathway expression in rats |
| Hits 422 Download times 270 Received:May 28, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.10.16 |
| Key Words
membrane-kidney No.1 formula;PI3K/Akt/mTOR signaling pathway;membranous nephropathy |
| Author Name | Affiliation | E-mail | | MENG Xi | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | DING Wei | Tianjin Hedong Traditional Chinese Medicine Hospital, Tianjin 300162, China | | | WANG Jianmei | Tianjin Beichen Hospital, Tianjin 300400, China | | | WANG Yaoguang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | wangyaoguang@163.com |
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| Abstract
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| [Objective] Observe the improvement effect of membranous kidney No.1 formula on renal pathology in rats with membranous nephropathy and its effect on the expression of autophagy pathway PI3K/Akt/mTOR related proteins. [Methods] Randomly divide the rats into a control group，a modeling group，a high-dose group of membranous kidney No.1 formula，a medium dose group，a low dose group，and a benazepril hydrochloride group. A membranous kidney rat model was established by injecting cationic bovine serum albumin（C-BSA） into the tail vein of rats，and the samples were taken by gavage. HE staining method was used to observe the pathological changes of rat kidney tissue；IgG immunofluorescence staining was used to observe the deposition of IgG in rats；Western blot was used to detect the expression of PI3K/Akt/mTOR signaling pathway related proteins and autophagy related protein LC3. [Results] After drug intervention，the 24-hour urinary protein，triglyceride，total cholesterol，high-density lipoprotein and low-density lipoprotein of rats in the membranous kidney No.1 formula group decreased and were lower than those in the model group，with statistical differences（P＜0.05）. Under the light microscope，HE staining showed that the overall structure of the normal group’s renal tissue was basically normal. In the membranous kidney model group，the glomerular capillary plexus was congested，the mesangial hyperplasia was observed，the basement membrane was thickened，some renal tubular cells were vacuolated，inflammatory cell infiltration was observed in the tissue，and eosinophil and IgG deposition were observed. After intervention with membranous kidney No.1 formula and benazepril hydrochloride，the pathological changes in the kidneys of rats were alleviated. The IgG deposition of rats in each group showed that compared with the normal group，the model group had significant IgG deposition and significantly increased IgG fluorescence expression（P＜0.05）. The IgG fluorescence expression of the benazepril hydrochloride group and the membranous kidney No.1 formula group decreased and was lower than the model group，with statistical differences（P＜0.05）.Western blot analysis showed that after drug intervention，the expression of PI3K-Akt signaling pathway related proteins decreased and the expression of LC3 increased in the benazepril hydrochloride group and the membranous kidney No.1 formula group rats，with statistical differences（P＜0.05）. [Conclusion] Membrane-kidney No.1 formula can improve renal pathological damage in rats. After intervention with Membrane-kidney No.1 formula，the expression of PI3K-Akt signaling pathway related proteins p-PI3K，p-Akt，p-mTOR protein was significantly reduced，while the expression of autophagy related protein LC3 was increased. Its molecular mechanism is related to the regulation of autophagy signaling pathway. |
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