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| Effect of overexpression of JAK1 on protection of fucoidan-exposed neurons from glucose-oxygen deprivation/reoxygenation injury |
| Hits 277 Download times 293 Received:July 10, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.10.18 |
| Key Words
fucoidan;mitophagy;JAK1/STAT3 pathway;PC12 cell;oxidative stress |
| Author Name | Affiliation | E-mail | | JU Hu | Affiliated Hospital of Qinghai University, Xining 810001, China | | | ZHAI Ting | Affiliated Hospital of Qinghai University, Xining 810001, China | | | DAWA Zhuoma | High Altitude Medicine Research Center of Qinghai University, Xining 810000, China | | | LIU Chuanchuan | Affiliated Hospital of Qinghai University, Xining 810001, China | 2017900006@qhu.edu.cn |
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| Abstract
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| [Objective] In this study，we investigated whether fucoidan protects neurons from oxidative damage and apoptosis caused by glucose-oxygen deprivation/reoxygenation injury（OGD/RP） by inhibiting JAK1/STAT3 signal pathway. [Methods] A rat model of glucose-oxygen deprivation/reoxygenation injury（OGD/RP） in PC12 cells was established，and the maximum concentration of fucoidan on OGD/RP model was selected by CCK8 method；apoptosis was detected by flow cytometry；ROS levels were determined by reactive oxygen species（ROS） fluorescent probe（DCFH-DA）；superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） contents were detected by enzyme-linked immunosorbent assay（ELISA）；mitochondrial membrane（MMP） potential changes were determined by JC-1 assay；mitophagy was observed by transmission electron microscopy；mitophagy，apoptosis and JAK1/STAT3 pathway and other related proteins were detected by Western blot. [Results] The 400 mg/kg fucoidan group under OGD/RP injury model was screened by CCK8 to have the strongest cell activity and proliferation ability，which was used for subsequent experimental research. Compared with the OGD/RP group，the contents of SOD and GSH-Px，the quantity of mitophagosomes，the MMP value and the relative expressions of PINK1，Parkin and Lc3II/Lc3I proteins in the cells of the OGD/RP+fucoidan group were increased. Apoptosis rate，apoptosis-related proteins，ROS content and the relative expression of p-JAK1/JAK1，p-STAT/STAT3 proteins were decreased. Compared with the OGD/RP+fucoidan group，the SOD，GSH-Px contents，the quantity of mitophagosomes，MMP value and the relative expressions of PINK1，Parkin and Lc3II/Lc3I proteins of the cells in the OGD/RP+fucoidan+pcDNA3.1-JAK1 group were decreased，while ROS content was increased. [Conclusion] Fucoidan can protect neurons from OGD/RP injury by inhibiting JAK/STAT signal pathway，reducing apoptosis and oxidative damage，and promoting mitophagy. |
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