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| Establishment of HPLC fingerprint and determination of multi-componentsof Qingre Lidan Tablet |
| Hits 965 Download times 914 Received:July 12, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.11.18 |
| Key Words
Qingre Lidan Tablet;HPLC;fingerprint;content determination |
| Author Name | Affiliation | E-mail | | QIAO Xiaoli | Tianjin Nankai Hospital, Tianjin 300100, China | | | CAO Ningning | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | WANG Qingguo | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Xiaojun | Tianjin Nankai Hospital, Tianjin 300100, China | | | ZHANG Haifang | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | 343697126@qq.com | | XIAO Xuefeng | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | kai1219@163.com |
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| Abstract
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| [Objective] To establish ahigh performance liquid chromatography(HPLC) fingerprint and a determination method of 14 components in Qingre Lidan Tablets, so as toevaluate the quality of Qingre Lidan Tablets. [Methods] Eclipse XDB-C18(5 μm, 4.6 mm×250 mm) was used for gradient elution with 0.1% phosphoric acid in water and acetonitrileas mobile phase. The column temperature was 35℃, the flow rate was 1.0 mL/min, and the detection wavelength was 294 nm. HPLC fingerprints of 15 batches of Qingre Lidan Tablets were established, and similarity analysis was conducted by using similarity evaluation software of Chinese medicine chromatographic fingerprint(2012), afterwords, calibrated the common peak and identified it, and on this basis, determined the content of the identified components. [Results] The similarity of different batches of Qingre Lidan Tablets was above 0.983. There were 62 common peaks in the fingerprints of 15 batches of samples, among which 14 components were identified, including Chlorogenic acid, Hyperoside, Quercetin, Kaempferol, Rutinum, Rosmarinic acid, Baicalin, Aloeemodin, Rhein, Emodin, Chrysophanic acid, Physcion, Magnolol, and Honokiol. The average content of them was (0.31±0.04)(0.04±0.01)(0.25±0.02)(0.20±0.06)(0.10±0.02)(0.23±0.05)(0.17±0.01)(0.19±0.02)(0.18±0.03)(0.56±0.08)(0.14±0.02)(1.98±0.17)(1.03±0.12)(0.65±0.04) mg/tablet, respectively. [Conclusion] The established HPLC fingerprint combined with multi-component content determination method for Qingre Lidan Tablets is sensitive and stable, and can be used for quality evaluation of Qingre Lidan Tablets. |
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