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| Mechanism of Yiqi Qufeng Formula in the treatment of diabetic nephropathy based on UHPLC-Q-Exactive Orbitrap MS and network pharmacology |
| Hits 570 Download times 422 Received:July 16, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.11.19 |
| Key Words
wind-based treatment;diabetic nephropathy;network pharmacology;UHPLC-Q-Exactive Orbitrap MS;molecular docking |
| Author Name | Affiliation | E-mail | | ZHU Liwei | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | WANG Chunguo | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China | | | FU Xiaozhe | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | HUANG Weijun | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | LI Xiaoran | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | ZHAO Jinxi | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | zhaojinximd@126.com |
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| Abstract
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| [Objective] To analyze the material basis and mechanism of Yiqi Qufeng Formula(YQQF) in the prevention and treatment of diabetic nephropathy(DN) by means of ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap MS) combined with network pharmacology and molecular docking technology. [Methods] The main chemical components of YQQF were identified by UHPLC-Q-Exactive Orbitrap MS, and the "main component-core target-signal pathway" network was constructed by network pharmacology. GO functional enrichment analysis and KEGG pathway enrichment analysis were conducted, and molecular docking between the main active components and core targets was conducted. [Results] A total of 115 chemical components were identified from YQQF and 40 core targets were obtained, mainly involving PI3K-Akt signaling pathway, lipid-arteriosclerosis, AGE-RAGE signaling pathway, chemokine signaling pathway and HIF-1 signaling pathway. Molecular docking results showed that pinocembrin, mosloflavone, quercetin, N-acetyldopamine dimer-1, N-acetyldopamine dimer-2 and other components had good binding activity with some core targets. [Conclusion] This study preliminarily identified the potential effective components of YQQF, providing ideas for further research on the "wind-based treatment" method and the mechanism of YQQF. |
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