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| Effect of hydroxysafflor yellow A(HSYA) on neuron against hypoxia/reoxygenation injury and its mechanism |
| Hits 378 Download times 432 Received:August 25, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.11.20 |
| Key Words
hydroxysafflor yellow A;neuron;hypoxia/reoxygenation;synaptic plasticity |
| Author Name | Affiliation | E-mail | | JIA Xiaoxu | Department of Pharmacy, Ordos Hospital of Traditional Chinese Medicine, Ordos 017010, China
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Yibo | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YUAN Qing | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Shuo | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHU Jinqiang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | zhujinqiang1860@163.com |
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| Abstract
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| [Objective] To introduce HSYA might act as a potential neuroprotective agent against the hypoxia/reoxygenation(H/R)injury in neuron. [Methods] Cortical neurons of Wistar rat were primary cultured in vitro. The cortical neurons were divided randomly into five groups:control, H/R and HSYA(5, 20, 80 μg/mL)+H/R. By observing the changes of cell viability and the releasing of LDH, proved the effect of HSYA on neuron injuried by H/R. The expressions of BDNF, GAP-43 and NogoA were studied. By using real-time RT-PCR and ELISA. [Results] Compared with control group, the cell viability decreased and the releasing of LDH increased significantly(P<0.01), the mRNA and protein expressions of BDNF and GAP-43 decreased significantly(P<0.05), the mRNA and protein expression of NogoA increased significantly(P<0.01) in H/R group. Compared with control group, three dosages of HSYA increased cell viability and reduced the releasing of LDH significantly(P<0.01), and increased the mRNA and protein expressions of BDNF and GAP-43, and decreased the mRNA and protein expressions of NogoA(P<0.05 or P<0.01). [Conclusion] HSYA could increase cell viability, and reduce the releasing of LDH, thus relieving the neuronal damage caused by H/R, which related to promoting BDNF expression. In addition, HSYA can also promote the neuronal synaptic plasticity by promoting the expression of GAP-43 mRNA and protein, and inhibiting the expression of Nogo-An mRNA and protein. |
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