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| Study on the effect of oridonin combined with immune factor TRAF4 on autophagy and apoptosis of non-small cell lung cancer cells and its mechanism |
| Hits 350 Download times 408 Received:August 10, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.11.21 |
| Key Words
oridonin;TRAF4;non-small cell lung cancer;autophagy;apoptosis |
| Author Name | Affiliation | E-mail | | LUO Shuai | Chongming Central Hospital Affiliated to Shanghai Medical College, Shanghai 202150, China | | | CHEN Yong | Chongming Central Hospital Affiliated to Shanghai Medical College, Shanghai 202150, China | 296368174@qq.com |
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| Abstract
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| [Objective] To investigate the effect of oridonin combined with immune factor TRAF4 on autophagy and apoptosis of non-small cell lung cancer cells and its mechanism. [Methods] Different concentrations of oridonin were selected to treat A549 cells, and then MTT method was used to detect cell viability. Culture A549 cells in vitro and divide the cells into control group, oridonin group, si-NC group(transfected with si-NC), si-TRAF4 group(transfected with si-TRAF4), oridonin+pcDNA group(after transfection of pcDNA, select the oridonin concentration of 20 μmol/L for treatment), oridonin+TRAF4 group(after transfection of TRAF4, select the oridonin concentration for treatment of 20 μmol/L). Flow cytometry was used to detect cell apoptosis;Western blot were used to detect the protein expression of Bax, Bcl-2, LC3Ⅱ/LC3Ⅰ, Beclin 1, ATG7 and TRAF4;qRT-PCR detection of TRAF4 mRNA expression. [Results] Cell viability was suppressed by different concentrations of oridonin. The cell apoptosis rate, Bax, Beclin 1, LC3Ⅱ/LC3Ⅰ, and ATG7 protein expression in oridonin group were significantly higher than those of the control group;TRAF4 mRNA and protein expression, and Bcl-2 protein expression were significantly lower than those of the control group. The expression of TRAF4 mRNA and protein, and the expression of Bcl-2 protein in the si-TRAF4 group were significantly lower than those in the si-NC group;the apoptosis rate, Bax, Beclin 1, LC3Ⅱ/LC3Ⅰ and ATG7 protein expression were significantly higher than those in the si-NC group. The expression of TRAF4 mRNA and protein and Bcl-2 protein in the oridonin + TRAF4 group was significantly higher than that of the oridonin+pcDNA group;the apoptotic rate, Bax, Beclin 1, LC3Ⅱ/LC3Ⅰ and ATG7 protein expression were significantly lower in the oridonin+pcDNA group. [Conclusion] Oridonin inhibits the apoptosis and autophagy of non-small cell lung cancer cells by down-regulating the expression of TRAF4. |
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